Detailed information NTG0415227

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GO012_RS04445
• Genome accession: NZ_CP047677
• Coordinates: 959640..960440 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KUH-KPNHVL1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 954640..965440

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GO012_RS04425 (GO012_04425)	dksA	955220..955675 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
GO012_RS04430 (GO012_04430)	sfsA	955838..956554 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
GO012_RS04435 (GO012_04435)	thpR	956554..957090 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
GO012_RS04440 (GO012_04440)	hrpB	957163..959592 (+)	2430	WP_012737409.1	ATP-dependent helicase HrpB	-
GO012_RS04445 (GO012_04445)	pilD	959640..960440 (-)	801	WP_004145898.1	prepilin peptidase	Machinery gene
GO012_RS04450 (GO012_04450)	-	960437..961183 (-)	747	WP_012737410.1	type II secretion system protein N	-
GO012_RS04455 (GO012_04455)	-	961173..961643 (-)	471	WP_141450063.1	type II secretion system protein M	-
GO012_RS04460 (GO012_04460)	gspL	961636..962829 (-)	1194	WP_012737412.1	type II secretion system protein GspL	-
GO012_RS04465 (GO012_04465)	gspK	962816..963802 (-)	987	WP_077250502.1	type II secretion system minor pseudopilin GspK	-
GO012_RS04470 (GO012_04470)	gspJ	963799..964395 (-)	597	WP_012737414.1	type II secretion system minor pseudopilin GspJ	-
GO012_RS04475 (GO012_04475)	gspI	964392..964757 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
GO012_RS04480 (GO012_04480)	gspH	964754..965269 (-)	516	WP_004183045.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=415227 GO012_RS04445 WP_004145898.1 959640..960440(-) (pilD) [Klebsiella pneumoniae subsp. pneumoniae strain KUH-KPNHVL1]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=415227 GO012_RS04445 WP_004145898.1 959640..960440(-) (pilD) [Klebsiella pneumoniae subsp. pneumoniae strain KUH-KPNHVL1]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425