Detailed information    

insolico Bioinformatically predicted

Overview


Name   sinI   Type   Regulator
Locus tag   GNE05_RS12915 Genome accession   NZ_CP047644
Coordinates   2646677..2646850 (+) Length   57 a.a.
NCBI ID   WP_014418369.1    Uniprot ID   I2C7P2
Organism   Bacillus sp. AM1(2019)     
Function   inhibit the expression of sinR (predicted from homology)   
Competence regulation

Genomic Context


Location: 2641677..2651850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNE05_RS12900 (GNE05_12900) gcvT 2642490..2643590 (-) 1101 WP_061890721.1 glycine cleavage system aminomethyltransferase GcvT -
  GNE05_RS12905 (GNE05_12905) - 2644014..2645684 (+) 1671 WP_021494309.1 SNF2-related protein -
  GNE05_RS12910 (GNE05_12910) - 2645706..2646500 (+) 795 WP_160182872.1 YqhG family protein -
  GNE05_RS12915 (GNE05_12915) sinI 2646677..2646850 (+) 174 WP_014418369.1 anti-repressor SinI family protein Regulator
  GNE05_RS12920 (GNE05_12920) sinR 2646884..2647219 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  GNE05_RS12925 (GNE05_12925) - 2647267..2648052 (-) 786 WP_007408329.1 TasA family protein -
  GNE05_RS12930 (GNE05_12930) - 2648117..2648701 (-) 585 WP_022552967.1 signal peptidase I -
  GNE05_RS12935 (GNE05_12935) tapA 2648673..2649344 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  GNE05_RS12940 (GNE05_12940) - 2649603..2649932 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  GNE05_RS12945 (GNE05_12945) - 2649973..2650152 (-) 180 WP_003153093.1 YqzE family protein -
  GNE05_RS12950 (GNE05_12950) comGG 2650209..2650586 (-) 378 WP_061862389.1 competence type IV pilus minor pilin ComGG Machinery gene
  GNE05_RS12955 (GNE05_12955) comGF 2650587..2650982 (-) 396 WP_061862390.1 competence type IV pilus minor pilin ComGF -
  GNE05_RS12960 (GNE05_12960) comGE 2650996..2651310 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  GNE05_RS12965 (GNE05_12965) comGD 2651294..2651731 (-) 438 WP_095061019.1 competence type IV pilus minor pilin ComGD Machinery gene

Sequence


Protein


Download         Length: 57 a.a.        Molecular weight: 6642.60 Da        Isoelectric Point: 9.8168

>NTDB_id=414876 GNE05_RS12915 WP_014418369.1 2646677..2646850(+) (sinI) [Bacillus sp. AM1(2019)]
MKNAKTEFSQLDKEWVELILEAQKANISLEEIRKYLLSNKKSSQHIQAARSHTVNPF

Nucleotide


Download         Length: 174 bp        

>NTDB_id=414876 GNE05_RS12915 WP_014418369.1 2646677..2646850(+) (sinI) [Bacillus sp. AM1(2019)]
ATGAAAAATGCAAAAACAGAATTTTCGCAATTAGATAAGGAATGGGTTGAGCTGATATTAGAAGCCCAAAAAGCAAATAT
CAGCCTAGAAGAAATACGCAAATACCTGCTTTCGAATAAAAAGTCTTCTCAACATATCCAGGCAGCCAGAAGTCATACCG
TAAATCCTTTCTGA

Domains


Predicted by InterproScan.

(11-37)


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB I2C7P2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sinI Bacillus subtilis subsp. subtilis str. 168

71.93

100

0.719


Multiple sequence alignment