Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   GNE05_RS08510 Genome accession   NZ_CP047644
Coordinates   1680344..1681243 (+) Length   299 a.a.
NCBI ID   WP_060561696.1    Uniprot ID   -
Organism   Bacillus sp. AM1(2019)     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1675344..1686243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNE05_RS08490 (GNE05_08490) - 1676034..1677737 (+) 1704 WP_160182661.1 glycosyl transferase -
  GNE05_RS08495 (GNE05_08495) - 1677734..1678015 (+) 282 WP_014417762.1 FlhB-like flagellar biosynthesis protein -
  GNE05_RS08500 (GNE05_08500) sucC 1678190..1679347 (+) 1158 WP_003154283.1 ADP-forming succinate--CoA ligase subunit beta -
  GNE05_RS08505 (GNE05_08505) sucD 1679376..1680278 (+) 903 WP_003154281.1 succinate--CoA ligase subunit alpha -
  GNE05_RS08510 (GNE05_08510) dprA 1680344..1681243 (+) 900 WP_060561696.1 DNA-processing protein DprA Machinery gene
  GNE05_RS08515 (GNE05_08515) topA 1681425..1683500 (+) 2076 WP_007409771.1 type I DNA topoisomerase -
  GNE05_RS08520 (GNE05_08520) trmFO 1683565..1684872 (+) 1308 WP_160182662.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  GNE05_RS08525 (GNE05_08525) xerC 1684942..1685859 (+) 918 WP_007409774.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32854.97 Da        Isoelectric Point: 7.8285

>NTDB_id=414868 GNE05_RS08510 WP_060561696.1 1680344..1681243(+) (dprA) [Bacillus sp. AM1(2019)]
MDQASRCLMVCSINQIISPSLLLKWWKADHSLSFLPDPHSLTVLSEGKTAPEAIFREIERKDPELDEVLSDYRREGITVI
PISSSRYPTWLKAIYDPPAVLYAKGNTLLLEKGRKIGIVGTRKPTEDGIKAVGHLSAELSKKGWVIVSGLASGIDGLSHK
ASIRAKGLTIGVIAGGFHHIYPRENLLLAEYMAEHHLLLSEHPPETKPKKWHFPMRNRIISGLSEGIVVVQGKEKSGSLI
TAYQALDQGREVFAVPGSIFNPYSGGPIKLIQEGAKAVLCAEDIDGELTARCVQYTEPF

Nucleotide


Download         Length: 900 bp        

>NTDB_id=414868 GNE05_RS08510 WP_060561696.1 1680344..1681243(+) (dprA) [Bacillus sp. AM1(2019)]
TTGGATCAAGCATCGCGCTGTTTAATGGTCTGCAGTATTAATCAAATCATTTCCCCGTCTCTTCTATTAAAATGGTGGAA
AGCTGATCATTCTCTGTCTTTTTTACCGGATCCGCATTCATTAACTGTTTTATCAGAAGGGAAAACAGCCCCGGAAGCAA
TTTTTCGGGAAATAGAGCGCAAGGATCCGGAACTTGATGAAGTTCTGTCCGATTACCGCCGCGAAGGCATCACTGTCATT
CCGATTTCATCAAGCCGCTATCCAACATGGCTTAAAGCGATTTATGATCCGCCGGCTGTCTTGTATGCAAAAGGGAACAC
GCTGCTTCTTGAAAAAGGCAGAAAAATCGGGATTGTAGGAACGCGGAAACCGACGGAAGACGGAATAAAAGCGGTTGGGC
ATCTTTCCGCCGAACTCTCAAAAAAAGGCTGGGTCATTGTAAGCGGGCTTGCATCCGGTATAGACGGATTGTCTCATAAG
GCGAGCATCAGGGCAAAAGGGCTTACGATCGGCGTGATAGCCGGCGGATTCCATCACATCTATCCCCGGGAAAATCTCCT
GTTAGCAGAATACATGGCTGAACACCATCTCCTACTCTCAGAACATCCTCCTGAAACAAAGCCGAAAAAATGGCACTTTC
CGATGAGAAACCGCATAATCAGCGGATTAAGTGAAGGAATTGTGGTCGTGCAGGGAAAAGAAAAAAGCGGTTCATTAATC
ACAGCTTACCAGGCTCTCGATCAAGGCAGAGAGGTATTTGCCGTTCCGGGTTCCATATTTAATCCATATTCCGGAGGACC
TATAAAACTCATTCAAGAAGGGGCGAAAGCTGTATTATGCGCAGAGGATATTGACGGAGAGCTGACCGCCCGATGCGTTC
AGTATACGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

70.903

100

0.709
  dprA Legionella pneumophila strain ERS1305867

40.426

94.314

0.381
  dprA Lactococcus lactis subsp. cremoris KW2

41.667

88.294

0.368

Multiple sequence alignment