Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GNE05_RS02115 Genome accession   NZ_CP047644
Coordinates   385084..385521 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus sp. AM1(2019)     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 380084..390521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNE05_RS02090 (GNE05_02090) - 381104..382240 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  GNE05_RS02095 (GNE05_02095) - 382255..382689 (+) 435 WP_160182375.1 RDD family protein -
  GNE05_RS02100 (GNE05_02100) - 382761..383084 (+) 324 WP_007409357.1 YckD family protein -
  GNE05_RS02105 (GNE05_02105) - 383188..384624 (+) 1437 WP_160182376.1 family 1 glycosylhydrolase -
  GNE05_RS02110 (GNE05_02110) nin/comJ 384665..385063 (-) 399 WP_014416873.1 competence protein ComJ Regulator
  GNE05_RS02115 (GNE05_02115) nucA/comI 385084..385521 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GNE05_RS02120 (GNE05_02120) hxlB 385852..386409 (-) 558 WP_021494205.1 6-phospho-3-hexuloisomerase -
  GNE05_RS02125 (GNE05_02125) hxlA 386406..387041 (-) 636 WP_160182377.1 3-hexulose-6-phosphate synthase -
  GNE05_RS02130 (GNE05_02130) - 387273..387635 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=414844 GNE05_RS02115 WP_003156588.1 385084..385521(-) (nucA/comI) [Bacillus sp. AM1(2019)]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=414844 GNE05_RS02115 WP_003156588.1 385084..385521(-) (nucA/comI) [Bacillus sp. AM1(2019)]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment