Detailed information NTG0414777

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GUY22_RS21385
• Genome accession: NZ_CP047633
• Coordinates: 4319025..4319825 (+)
• Length: 266 a.a.
• NCBI ID: WP_032414527.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain K2606
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4314025..4324825

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GUY22_RS21350 (GUY22_20635)	gspH	4314181..4314696 (+)	516	WP_032435598.1	type II secretion system minor pseudopilin GspH	-
GUY22_RS21355 (GUY22_20640)	gspI	4314693..4315058 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
GUY22_RS21360 (GUY22_20645)	gspJ	4315055..4315651 (+)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
GUY22_RS21365 (GUY22_20650)	gspK	4315648..4316634 (+)	987	WP_004213439.1	type II secretion system minor pseudopilin GspK	-
GUY22_RS21370 (GUY22_20655)	gspL	4316621..4317814 (+)	1194	WP_004213440.1	type II secretion system protein GspL	-
GUY22_RS21375 (GUY22_20660)	-	4317807..4318292 (+)	486	WP_032411549.1	type II secretion system protein M	-
GUY22_RS21380 (GUY22_20665)	-	4318282..4319028 (+)	747	WP_009309263.1	type II secretion system protein N	-
GUY22_RS21385 (GUY22_20670)	pilD	4319025..4319825 (+)	801	WP_032414527.1	prepilin peptidase	Machinery gene
GUY22_RS21390 (GUY22_20675)	hrpB	4319873..4322302 (-)	2430	WP_032411546.1	ATP-dependent helicase HrpB	-
GUY22_RS21395 (GUY22_20680)	thpR	4322375..4322911 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
GUY22_RS21400 (GUY22_20685)	sfsA	4322911..4323627 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
GUY22_RS21405 (GUY22_20690)	dksA	4323790..4324245 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=414777 GUY22_RS21385 WP_032414527.1 4319025..4319825(+) (pilD) [Klebsiella pneumoniae strain K2606]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=414777 GUY22_RS21385 WP_032414527.1 4319025..4319825(+) (pilD) [Klebsiella pneumoniae strain K2606]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421