Detailed information NTG0413871

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GT391_RS14155
• Genome accession: NZ_CP047495
• Coordinates: 3168930..3169775 (+)
• Length: 281 a.a.
• NCBI ID: WP_010681639.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain 1692
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3163930..3174775

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GT391_RS14125 (GT391_14125)	gspI	3164322..3164687 (+)	366	WP_039284733.1	type II secretion system minor pseudopilin GspI	-
GT391_RS14130 (GT391_14130)	gspJ	3164687..3165337 (+)	651	WP_072014215.1	type II secretion system minor pseudopilin GspJ	-
GT391_RS14135 (GT391_14135)	gspK	3165334..3166320 (+)	987	WP_010681643.1	type II secretion system minor pseudopilin GspK	-
GT391_RS14140 (GT391_14140)	gspL	3166317..3167594 (+)	1278	WP_010681642.1	type II secretion system protein GspL	-
GT391_RS14145 (GT391_14145)	gspM	3167591..3168085 (+)	495	WP_010681641.1	type II secretion system protein GspM	-
GT391_RS14150 (GT391_14150)	-	3168075..3168821 (+)	747	WP_010681640.1	type II secretion system protein N	-
GT391_RS14155 (GT391_14155)	pilD	3168930..3169775 (+)	846	WP_010681639.1	prepilin peptidase	Machinery gene
GT391_RS14160 (GT391_14160)	ypdK	3169936..3170010 (-)	75	WP_099606229.1	membrane protein YpdK	-
GT391_RS14165 (GT391_14165)	-	3170368..3171393 (-)	1026	WP_010681638.1	LLM class flavin-dependent oxidoreductase	-
GT391_RS14170 (GT391_14170)	-	3171704..3172768 (+)	1065	WP_010681637.1	ABC transporter substrate-binding protein	-
GT391_RS14175 (GT391_14175)	-	3172765..3173784 (+)	1020	WP_010681636.1	FecCD family ABC transporter permease	-
GT391_RS14180 (GT391_14180)	-	3173786..3174556 (+)	771	WP_010681635.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31428.35 Da
• Isoelectric Point: 5.7656

>NTDB_id=413871 GT391_RS14155 WP_010681639.1 3168930..3169775(+) (pilD) [Pectobacterium brasiliense strain 1692]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGIADSDPDTRYNLWWPPSSCPHCQQAI
VVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWIGLLFNLSETFVPLSDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGFNL

Nucleotide

• Length: 846 bp

>NTDB_id=413871 GT391_RS14155 WP_010681639.1 3168930..3169775(+) (pilD) [Pectobacterium brasiliense strain 1692]
GTGGACGATTTAAGGGAATTTGCACAGCTGTTTCCGGCGTGGTGGTTTGGATCGCTGGGCGTGTTGGGATTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCATAGCCGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCTTGTCCGCACTGCCAGCAGGCTATT
GTGGTGAAGGATAACATCCCGCTATTCAGTTGGTTATGGCTGCGTGGCCGCTCCCGCTGCTGTCATCAATCAGTATCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGCTGCCCGGTATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCATTCCTGTTGGTGCTGACCGTTATTGATATCAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATAGGGCTGCTGTTTAATCTGTCGGAGACATTTGTCCCATTGAGTGATGCCGTAGTCGGCGC
GATGGCCGGTTATTTATCTCTGTGGCTGCTTTATTGGGTATTCAAATACGCAACGGGCAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAACCTGGTTCTGGTGGCGGCGCTGAGC
GGGTTGGTTGTTACTCTCGTCTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATGGGCGGTGTGTTTGGCGTGATAATGAACGGATTCAATCTGTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.923	96.441	0.491
pilD	Neisseria gonorrhoeae MS11	47.126	92.883	0.438
pilD	Vibrio campbellii strain DS40M4	44.981	95.73	0.431
pilD	Acinetobacter baumannii D1279779	41.758	97.153	0.406
pilD	Acinetobacter nosocomialis M2	41.392	97.153	0.402