Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GO994_RS02445 Genome accession   NZ_CP047376
Coordinates   467359..469065 (-) Length   568 a.a.
NCBI ID   WP_088814128.1    Uniprot ID   -
Organism   Aeromonas salmonicida subsp. salmonicida strain J410     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 462359..474065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO994_RS02415 (GO994_02415) mutT 462969..463376 (+) 408 WP_005314168.1 8-oxo-dGTP diphosphatase MutT -
  GO994_RS02420 (GO994_02420) yacG 463453..463647 (-) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  GO994_RS02425 (GO994_02425) zapD 463657..464379 (-) 723 WP_005314166.1 cell division protein ZapD -
  GO994_RS02430 (GO994_02430) coaE 464417..465031 (-) 615 WP_042468303.1 dephospho-CoA kinase -
  GO994_RS02435 (GO994_02435) pilD 465051..465926 (-) 876 WP_005314162.1 A24 family peptidase Machinery gene
  GO994_RS02440 (GO994_02440) - 465990..467230 (-) 1241 Protein_450 type II secretion system F family protein -
  GO994_RS02445 (GO994_02445) pilB 467359..469065 (-) 1707 WP_088814128.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  GO994_RS02450 (GO994_02450) tapA 469069..469497 (-) 429 WP_005314158.1 type IVa pilus major pilin TapA -
  GO994_RS02455 (GO994_02455) nadC 469832..470689 (-) 858 WP_005314155.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GO994_RS02460 (GO994_02460) - 470694..471164 (-) 471 WP_201449072.1 TIGR02281 family clan AA aspartic protease -
  GO994_RS02465 (GO994_02465) ampD 471314..471886 (+) 573 WP_005314151.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GO994_RS02470 (GO994_02470) pdhR 472163..472927 (+) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62562.09 Da        Isoelectric Point: 5.4384

>NTDB_id=413093 GO994_RS02445 WP_088814128.1 467359..469065(-) (pilB) [Aeromonas salmonicida subsp. salmonicida strain J410]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESELDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNITEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=413093 GO994_RS02445 WP_088814128.1 467359..469065(-) (pilB) [Aeromonas salmonicida subsp. salmonicida strain J410]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCAGA
CCCGACCAACGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTCCATACCGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCTGGATGCCCTCGGCATGGAGGAGATCGACGAGTCGGAGATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCCGATGACGATGCCCCCATCGTCAA
ATACATCAATAAAATCATGATGGATGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGCTTTCGTATAGACGGCATACTGCACGAAATAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCAGAGCGACGGCTGCCCCAGGATGGTCGCATCAAGTTAAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CAGCGCGGCTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGCACAGTATCTGCGTGCCCTCTCCAAGCCTCAG
GGCATGATACTGGTGACGGGCCCGACCGGCTCTGGCAAGACGGTCTCTCTCTATACCGGCCTCAATATTCTCAACATCAC
AGAGGTGAACATCTCAACCGCCGAAGATCCGGTGGAGATCAACCTGCCAGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGTTGACCTTCGCCAGCGCCCTGCGATCCTTTCTTCGCCAGGATCCCGACGTTGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCAGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTCCCGGCCTTCAATATCGCCTCTTCGGTGACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCACCGGAAGTGGTGCCCGAAGCTGAATTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAGCCAGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATTATGCAAGGAGCCAACTCCCTGCAAATTGCAG
CGATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCTTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.24

99.648

0.54
  pilB Vibrio cholerae strain A1552

53.805

99.472

0.535
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.601

98.944

0.511
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

38.336

97.359

0.373

Multiple sequence alignment