Detailed information NTG0413082

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GO992_RS15705
• Genome accession: NZ_CP047374
• Coordinates: 3303124..3303999 (-)
• Length: 291 a.a.
• NCBI ID: WP_005314162.1
• Uniprot ID: A2T195
• Organism: Aeromonas salmonicida subsp. salmonicida strain J409
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3298124..3308999

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GO992_RS15680 (GO992_15680)	-	3299849..3301033 (+)	1185	WP_102665304.1	tetratricopeptide repeat protein	-
GO992_RS15685 (GO992_15685)	mutT	3301042..3301449 (+)	408	WP_005314168.1	8-oxo-dGTP diphosphatase MutT	-
GO992_RS15690 (GO992_15690)	yacG	3301526..3301720 (-)	195	WP_005314167.1	DNA gyrase inhibitor YacG	-
GO992_RS15695 (GO992_15695)	zapD	3301730..3302452 (-)	723	WP_005314166.1	cell division protein ZapD	-
GO992_RS15700 (GO992_15700)	coaE	3302490..3303104 (-)	615	WP_042468303.1	dephospho-CoA kinase	-
GO992_RS15705 (GO992_15705)	pilD	3303124..3303999 (-)	876	WP_005314162.1	A24 family peptidase	Machinery gene
GO992_RS15710 (GO992_15710)	-	3304063..3305303 (-)	1241	Protein_3039	type II secretion system F family protein	-
GO992_RS15715 (GO992_15715)	pilB	3305432..3307138 (-)	1707	WP_088814128.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene
GO992_RS15720 (GO992_15720)	tapA	3307142..3307570 (-)	429	WP_005314158.1	type IVa pilus major pilin TapA	-
GO992_RS15725 (GO992_15725)	nadC	3307905..3308762 (-)	858	WP_005314155.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32500.78 Da
• Isoelectric Point: 6.9383

>NTDB_id=413082 GO992_RS15705 WP_005314162.1 3303124..3303999(-) (pilD) [Aeromonas salmonicida subsp. salmonicida strain J409]
MTLLLELAHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFSSDTPQPEDDERYNLMVPRSCCPRCNH
PITALENIPLLSWLWLKGRCRGCQAAISARYPLVELLTALLSVVVAMTLTPGWGTLAALLLTWVLVALTFIDLDKMLLPD
QLTLPLLWGGLLFNLLGGYVPLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSS
LVGAIFGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWYLSTIL

Nucleotide

• Length: 876 bp

>NTDB_id=413082 GO992_RS15705 WP_005314162.1 3303124..3303999(-) (pilD) [Aeromonas salmonicida subsp. salmonicida strain J409]
ATGACACTTCTTCTCGAGCTGGCACACGGCTTGCCATGGCTCTACTTCTCGCTGGTTTTTCTCTTCTCCCTGATGATCGG
CAGCTTTCTCAACGTGGTGATCCACCGCCTGCCCATCATGCTGGAACGGGAGTGGCAGGCCGAATATCGCAGCTATTTCA
GTTCAGACACGCCGCAGCCAGAAGATGACGAGCGCTACAATCTGATGGTGCCGCGCTCCTGCTGCCCTCGCTGCAATCAC
CCCATAACGGCCTTGGAAAACATTCCCCTGCTGAGCTGGCTCTGGCTCAAAGGTCGCTGCCGGGGCTGCCAGGCCGCCAT
TTCGGCTCGCTATCCGCTGGTGGAGCTGCTGACCGCGCTGCTCTCTGTGGTGGTTGCCATGACGCTCACCCCTGGCTGGG
GCACGCTGGCTGCCCTCCTGCTGACCTGGGTGCTGGTGGCGCTCACCTTTATCGATCTGGACAAGATGCTGCTGCCGGAC
CAGCTCACCTTGCCGCTACTGTGGGGCGGGCTGCTGTTCAACCTGCTGGGCGGATACGTGCCTCTCGGCGATGCTGTCAT
CGGCGCCATGGCAGGCTATCTGGTGCTGTGGAGCCTCTACTGGGCCTTCAAGCTGCTTACCGGCAAGGAGGGCATGGGCT
ATGGGGACTTCAAACTGCTGGCAGCGCTGGGAGCCTGGCTTGGCTGGCAGGCGCTGCCCATAGTACTGCTGCTCTCTTCT
CTGGTCGGCGCCATCTTCGGCATTGGCCTCATTTTGCTGCGCAACCATCACCAGAGCAAACCCATCCCCTTTGGCCCCTA
TCTCGCCATCGCAGGCTGGATCGCACTGCTCTGGGGCGATAGCATCACCCGCTGGTATCTCTCCACTATCCTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-248)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-128)

3D structure

• AlphaFold DB: A2T195

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	60.764	98.969	0.601
pilD	Vibrio campbellii strain DS40M4	55.556	98.969	0.55
pilD	Acinetobacter baumannii D1279779	55.39	92.44	0.512
pilD	Acinetobacter nosocomialis M2	55.39	92.44	0.512
pilD	Neisseria gonorrhoeae MS11	50.746	92.096	0.467