Detailed information NTG0413024

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: GSN03_RS05920
• Genome accession: NZ_CP047366
• Coordinates: 1168971..1172696 (+)
• Length: 1241 a.a.
• NCBI ID: WP_212089592.1
• Uniprot ID: -
• Organism: Bacillus nitratireducens strain BM02
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1163971..1177696

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GSN03_RS05905 (GSN03_05840)	-	1164129..1164722 (+)	594	WP_000347513.1	TVP38/TMEM64 family protein	-
GSN03_RS05910 (GSN03_05845)	lepB	1164779..1165342 (+)	564	WP_000751912.1	signal peptidase I	-
GSN03_RS05915 (GSN03_05850)	addB	1165459..1168974 (+)	3516	WP_212089590.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
GSN03_RS05920 (GSN03_05855)	addA	1168971..1172696 (+)	3726	WP_212089592.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
GSN03_RS05925 (GSN03_05860)	-	1172720..1172998 (+)	279	Protein_1095	RNA polymerase alpha subunit C-terminal domain-containing protein	-
GSN03_RS05930 (GSN03_05865)	-	1173149..1173856 (+)	708	WP_212089594.1	DNA alkylation repair protein	-
GSN03_RS05935 (GSN03_05870)	-	1174126..1174611 (+)	486	WP_002149902.1	hypothetical protein	-
GSN03_RS05940 (GSN03_05875)	gerPF	1174694..1174909 (-)	216	WP_001141570.1	spore germination protein GerPF	-
GSN03_RS05945 (GSN03_05880)	-	1174952..1175338 (-)	387	WP_212089596.1	spore germination protein GerPE	-
GSN03_RS05950 (GSN03_05885)	gerPD	1175353..1175547 (-)	195	WP_001052805.1	spore germination protein GerPD	-
GSN03_RS05955 (GSN03_05890)	gerPC	1175554..1176168 (-)	615	WP_001070768.1	spore germination protein GerPC	-
GSN03_RS05960 (GSN03_05895)	gerPB	1176233..1176439 (-)	207	WP_001012507.1	spore germination protein GerPB	-
GSN03_RS05965 (GSN03_05900)	gerPA	1176454..1176675 (-)	222	WP_001111190.1	spore germination protein GerPA	-
GSN03_RS05970 (GSN03_05905)	-	1176772..1176951 (-)	180	WP_000462854.1	aspartyl-phosphate phosphatase Spo0E family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142664.95 Da
• Isoelectric Point: 4.9214

>NTDB_id=413024 GSN03_RS05920 WP_212089592.1 1168971..1172696(+) (addA) [Bacillus nitratireducens strain BM02]
MIENWPQKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDGPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVKAYDVEGKTIEDLVYASYLLEDVKFQLETAEKHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAACRSWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVKLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRQVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHGEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPGEIYEYDTSWK
VAVVEGKDLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLMWEYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIARMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGKKGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPTL
EDRYKVQLSLYAKALEKSLKHPVKEKCLYFFDGNHVVSIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=413024 GSN03_RS05920 WP_212089592.1 1168971..1172696(+) (addA) [Bacillus nitratireducens strain BM02]
ATGATAGAAAATTGGCCTCAGAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCAGCAGCTGGATCAGGGAAAACGGCAGTATTAGTTGAACGGATTATTAAAAAGATTATAAGTG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGGACCAGGATCGCAGCATATAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCTATTTCTACCATTCATTCATTTTGTTTACAAGTGATTAGAGGATACTACTACATGCTTGATGTCGATCCTCGTTTTC
GTATTGCAAACCAAACAGAAAATGAGTTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGTTATACGAGTGACCGTAGTGACGATGACTTACAACGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAGAAGTGGCTTGATAAATTAGTAAAAGCATACGACGTTGAAGGAA
AGACGATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAAAAGCATATT
CGTAAAGCGACTGAACTCGCAATGCTTCCTGATGGTCCAGCGCCTCGCGTTGAAACCTTACAAGCAGATTTAGCTTTACT
TGGAACGTTATCCTCAGCAGCTTGTCGATCGTGGACAAGCGTTTATGAAGCAATGCAAAATGTATCGTGGCAAACGCTAA
AGCGTATTAAGAAAAGTGATTACAACGAAGATGTTGTAAAACAAGTAGATTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAAAAATTACAAGAAGAGCTATTTAGCCGCAGACCTGAAAGTTTCTTGCGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATTAAACGAGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGCGAGCAAAGTGAAGACGGTGAAATGAAGCCGTCGGCAGTAGCGCTGCAA
TATCGTAATAAATTTGCTGAAGTACTAGTTGATGAATATCAAGATACGAACTTCGTACAGGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATTTATCGTTTCCGACTAGCAG
AGCCTGGTTTATTCTTAGGAAAATATAAACGTTTCACACAAGAAGGATCGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGCTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGATTATGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAGCTATTATGTA
TTCAGCAAACAGAAGAAGAAGTGCAAGACGGAGAAGAAGGTGCCGAAGTAGAAAAAGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCAATGGTAGATTCAGGTTATGAAGTGTATGATCGTAAAACTGATAGTATGCGCCAAGTACAATA
CCGTGATTTCGTTATTTTACTTCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATATGCTGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGTGTTATCGATAAT
CCGATGCAGGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATCGTTGGATTAAACGACGAAGAACTTGCGACGCTTCG
TGCTCATGGAAAGAAAGGTTCATTTTATGAAGTGATGAGTTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAACAAGAAC
TGCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGGTGGCGTGAATTTGCGCGTCAACAATCCCTTTCCGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGCTATTACGACTTTGTCGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGCGCGAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGAGATGATATGGGAACGGCGAGAGCTCTTGGAGAACAAGAAGACGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTCGTATTTGTAGCTGGACTTGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGCTTCTTACTTCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATATGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTCGATGCAAGAGAGCA
TGGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTGGAATTAGGACAAGGAAATATTCCAGGTGAAATTTATGAATATGATACGAGCTGGAAA
GTAGCAGTTGTTGAAGGGAAAGATCTACTTGCACCAGAACCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTGTACGATAGATTAATGTGGGAATACGGGTATGAGG
ACGCAACATCGCACCGTGCGAAACAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGCAGCGATAATGCC
TTTATTAAAAAACTTCGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGCTAACGTATGCAGAGCGAGG
GACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAACAAATTGCAA
GAATGGTAAATAAAGAACTATTAACATTTGAACAAGCTGAAGAAATAGCGATTGAAAAGGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCAGCAAAAAGTGTTGAGCGCGAAGTACCATTTACGATGATGCTTTCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGAAGAAAGGCGAATCAATACTTGTCCAAGGGGTTATCGACTGTATGATCGAAGAGGAAGACG
GAATTACTTTAATCGACTTTAAAACAGATACGATTGAAGGAAAGTTTCCAGGCGGATTTGATCAAGCGAAACCAACTTTA
GAAGACCGATACAAAGTACAGCTTTCACTATATGCAAAAGCACTAGAGAAAAGCTTAAAACATCCTGTGAAAGAGAAGTG
TTTATACTTCTTTGATGGGAATCATGTTGTAAGTATTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.408	100	0.537