Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   GSN03_RS05915 Genome accession   NZ_CP047366
Coordinates   1165459..1168974 (+) Length   1171 a.a.
NCBI ID   WP_212089590.1    Uniprot ID   -
Organism   Bacillus nitratireducens strain BM02     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1160459..1173974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSN03_RS05875 (GSN03_05810) - 1160541..1161023 (-) 483 WP_212089588.1 DUF6584 family protein -
  GSN03_RS05880 (GSN03_05815) cspA 1161304..1161507 (+) 204 WP_000218989.1 RNA chaperone/antiterminator CspA -
  GSN03_RS05885 (GSN03_05820) - 1161612..1161887 (+) 276 WP_000970280.1 hypothetical protein -
  GSN03_RS05890 (GSN03_05825) - 1161914..1162099 (-) 186 WP_000391586.1 hypothetical protein -
  GSN03_RS05895 (GSN03_05830) - 1162382..1162963 (+) 582 WP_000444433.1 competence protein ComK -
  GSN03_RS05900 (GSN03_05835) - 1163208..1163804 (+) 597 Protein_1090 DUF4306 domain-containing protein -
  GSN03_RS05905 (GSN03_05840) - 1164129..1164722 (+) 594 WP_000347513.1 TVP38/TMEM64 family protein -
  GSN03_RS05910 (GSN03_05845) lepB 1164779..1165342 (+) 564 WP_000751912.1 signal peptidase I -
  GSN03_RS05915 (GSN03_05850) addB 1165459..1168974 (+) 3516 WP_212089590.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  GSN03_RS05920 (GSN03_05855) addA 1168971..1172696 (+) 3726 WP_212089592.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  GSN03_RS05925 (GSN03_05860) - 1172720..1172998 (+) 279 Protein_1095 RNA polymerase alpha subunit C-terminal domain-containing protein -
  GSN03_RS05930 (GSN03_05865) - 1173149..1173856 (+) 708 WP_212089594.1 DNA alkylation repair protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134351.40 Da        Isoelectric Point: 5.1506

>NTDB_id=413023 GSN03_RS05915 WP_212089590.1 1165459..1168974(+) (addB) [Bacillus nitratireducens strain BM02]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEEVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLSDSEYVKGAEIYIDGFHSFSPQELEIVRGLMKLGTRVTITLTIDE
KTLAQPVNELDLFYETTLTYERIKQVAREEKIEIEKTIPLMEQPRFHSQALAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDVAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDASEMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRVRAEENGDFLFATDHEQVWEEVMNLLDTFVEMLGEEK
MSLSMFTEVMSVGLEALQFANIPLSLDQVLVANIDHSRLSDIKATFIIGVNEGVIPAAPMDEGMLSDEERNVLTAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKESFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSTLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFALPNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHSFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=413023 GSN03_RS05915 WP_212089590.1 1165459..1168974(+) (addB) [Bacillus nitratireducens strain BM02]
ATGTCACTTCGATTTGTGATTGGTAGAGCTGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGTCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGGA
GTGAAGAGGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCGCGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCCGGTGTACATATGTTACTTCGTAAAATTGTAGAATCTCGTAAAGATGGATT
ATCGGTGTTTCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTATA
ATGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGTAGTGCAGAGCAAAAGCTGCTAGCG
AATAAAGTGTATGATTTACAGTTATTATATGATGATTTCGAACGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CTTACAATTATTAATTGAGAAGCTTTCAGACTCTGAGTATGTAAAAGGCGCGGAAATTTATATAGATGGATTCCATTCAT
TTTCCCCTCAAGAGCTAGAAATTGTTAGAGGGTTAATGAAATTAGGAACTAGAGTAACAATAACATTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTGAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAACGAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCGCTGATGGAACAGCCGCGTTTTCATTCGCAAGCATTAGCGCATT
TAGAAACGCACTACGAAGCACGTCCAAATGAAAAATTTCATGGTGAAGCGAGTGTGACAATCAGTACAGCAGCTAATTTA
CGAGCTGAAGTAGAAGGTGTAGCTCGTGAAATTAGAAGACTTGTGGCGGATGAAGACTATCGTTACCGAGATGTCGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGACGTAATGCGAACGTTATTTACGGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCAATGTCACATCATCCGCTAGTAGAATGTATTCGTTCTGCTTTAGAGATTATTAGTGGGAATTGGCGTTAT
GATGCAGTATTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGATGTAAGAAAAGAAGCGATGCGCGAAGAGATGGA
TGAGTTCGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCGTGGATGTATCGTCGTT
ATCGTTCTCTTGATGATGCGAGCGAAATGATAACAGACAGTGAGCGTGAAATGGAAGAGAAAATAAATCGACTTCGCGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCTGGAACAGTTATGCAAATGTGCGAAGCGGTATA
TTTATTTTTAGAGGAGCTTGACGTTCCGAAAAAACTAGAAGAATTACGTGTTCGTGCAGAAGAAAATGGAGATTTCTTAT
TTGCGACAGACCATGAGCAAGTATGGGAAGAAGTGATGAATCTTCTTGATACGTTCGTTGAGATGCTTGGTGAGGAGAAA
ATGTCACTATCTATGTTCACGGAGGTTATGTCGGTAGGTCTTGAGGCACTTCAATTTGCGAACATTCCGCTGTCACTAGA
TCAAGTGTTAGTTGCTAATATTGATCATTCTAGATTATCAGATATTAAAGCGACGTTCATTATTGGAGTAAACGAAGGTG
TGATTCCAGCAGCGCCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAAATGTTCTCACCGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTACTAGAAGAACAGTTCGTTATGTATCAAATGGTGACGAGAGCATCTGAGAAGTTATA
CATTTCTTGTCCGCTTGCAGATGAGGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGAATCATTTATTACAAATGACGTAAATGACTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTGACATTGTCTTATGTCATGCAGCAGCTGCAAACATGGAAGCGATACGGATTTGAAGGTAATTTAGACTTTTGGTG
GGATGTATATAACTTCTACGTTACTTCCGATGAATGGAAGCAAAAAAGTAGCCGTGTGTTATCAAGTTTATTCTACCGAA
ATCGTGCACAGAAATTAAGTACAGCTGTAAGTAGGGATTTATATGGAGATACAATTAAGGGAAGCGTTTCTCGTATGGAA
TTATTTAACCGTTGTGCGTACGCTCATTTCGCGCAGCACGGTTTATCGCTAAGAGAGCGTGATATTTTCAAACTTGATGC
GCCAGATATCGGTGAACTATTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAACCGTACTTGGGCAG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTACTTTAGTAATAGAAGAAATTGCACCATTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGGACATCACTTATTCTTCGTGAACA
TGCGAAGTCTAGCGGTTTTGTACCAGTTGATTTAGAAGTACCGTTCGGTATGGGCGGGACTGGATCGCTTCCGCCAATGG
AATTTGCGTTGCCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGACAAGGCGGAAGATGAGAGTGGT
ACGTTCCTTCGTATTATTGACTATAAATCAAGTTCGAAAGCGTTAGATTTAACAGAAGTGTATTACGGACTCGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGTGGCACTGCATCACCGGCGGGTG
TATTGTATTTCCATATTCATAACCCGATTGTTGAGATGAAAGGCGATGCATCTGAAGAAGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGGCTCGTACTAGGAGATGCTGATGTTGTTCGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTTTGAAAAAAGATGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAACAAGAATTTA
ACGTACTTCAAAAATATGTACACCACTCGTTTGAAAATATCGGAAAAGACATCACAGAAGGTGTCATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCTTGTACGTTCTGTAACTTTAAATCTGTTTGTCAGTTTGATGAATCACTTGAAGATAA
TCAATTCCGTACATTAAAAGATATGAAAGATAGCGAAGCAATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.148

100

0.493

Multiple sequence alignment