Detailed information NTG0412941

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GSY73_RS22435
• Genome accession: NZ_CP047360
• Coordinates: 4553629..4554429 (+)
• Length: 266 a.a.
• NCBI ID: WP_023322076.1
• Uniprot ID: -
• Organism: Klebsiella variicola strain AHKv-S01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4548629..4559429

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GSY73_RS22400 (GSY73_22410)	gspH	4548782..4549297 (+)	516	WP_167341439.1	type II secretion system minor pseudopilin GspH	-
GSY73_RS22405 (GSY73_22415)	gspI	4549294..4549659 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
GSY73_RS22410 (GSY73_22420)	gspJ	4549656..4550252 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
GSY73_RS22415 (GSY73_22425)	gspK	4550249..4551229 (+)	981	WP_167341440.1	type II secretion system minor pseudopilin GspK	-
GSY73_RS22420 (GSY73_22430)	gspL	4551222..4552418 (+)	1197	WP_080835738.1	type II secretion system protein GspL	-
GSY73_RS22425 (GSY73_22435)	-	4552411..4552896 (+)	486	WP_012968921.1	type II secretion system protein M	-
GSY73_RS22430 (GSY73_22440)	-	4552886..4553632 (+)	747	WP_048267462.1	type II secretion system protein N	-
GSY73_RS22435 (GSY73_22445)	pilD	4553629..4554429 (+)	801	WP_023322076.1	prepilin peptidase	Machinery gene
GSY73_RS22440 (GSY73_22450)	hrpB	4554476..4556905 (-)	2430	WP_167341441.1	ATP-dependent helicase HrpB	-
GSY73_RS22445 (GSY73_22455)	thpR	4556978..4557514 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
GSY73_RS22450 (GSY73_22460)	sfsA	4557514..4558230 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
GSY73_RS22455 (GSY73_22465)	dksA	4558393..4558848 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29445.19 Da
• Isoelectric Point: 8.7151

>NTDB_id=412941 GSY73_RS22435 WP_023322076.1 4553629..4554429(+) (pilD) [Klebsiella variicola strain AHKv-S01]
MTILTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=412941 GSY73_RS22435 WP_023322076.1 4553629..4554429(+) (pilD) [Klebsiella variicola strain AHKv-S01]
ATGACGATATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425