Detailed information NTG0412563

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GR140_RS03385
• Genome accession: NZ_CP047311
• Coordinates: 745096..745962 (-)
• Length: 288 a.a.
• NCBI ID: WP_003247536.1
• Uniprot ID: -
• Organism: Pseudomonas putida strain YC-AE1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 740096..750962

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GR140_RS03350 (GR140_03360)	-	740570..741865 (-)	1296	WP_003247549.1	NAD(P)/FAD-dependent oxidoreductase	-
GR140_RS03355 (GR140_03365)	-	741950..742123 (-)	174	WP_008091951.1	DUF3094 domain-containing protein	-
GR140_RS03360 (GR140_03370)	-	742256..742885 (-)	630	WP_003247546.1	DUF1780 domain-containing protein	-
GR140_RS03365 (GR140_03375)	-	742941..743372 (-)	432	WP_012051128.1	hypothetical protein	-
GR140_RS03370 (GR140_03380)	-	743459..744166 (+)	708	WP_014755237.1	energy-coupling factor ABC transporter permease	-
GR140_RS03375 (GR140_03385)	yacG	744276..744476 (-)	201	WP_003247540.1	DNA gyrase inhibitor YacG	-
GR140_RS03380 (GR140_03390)	coaE	744473..745096 (-)	624	WP_004575721.1	dephospho-CoA kinase	-
GR140_RS03385 (GR140_03395)	pilD	745096..745962 (-)	867	WP_003247536.1	A24 family peptidase	Machinery gene
GR140_RS03390 (GR140_03400)	-	745965..747173 (-)	1209	WP_054888521.1	type II secretion system F family protein	-
GR140_RS03395 (GR140_03405)	-	747390..747800 (+)	411	WP_212628271.1	pilin	-
GR140_RS03405 (GR140_03415)	-	748109..749432 (+)	1324	Protein_650	integrase arm-type DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31808.87 Da
• Isoelectric Point: 7.9101

>NTDB_id=412563 GR140_RS03385 WP_003247536.1 745096..745962(-) (pilD) [Pseudomonas putida strain YC-AE1]
MTLWALLAEQPAYFLTLATVLGLLVGSFINVLVYRLPIMLERQWQREAQEVLGLPTTQHPRFDLCLPASRCPHCAHRIRA
WENIPVISYLALGGRCSSCKNRISLRYPVVEVASALLSLVVAWRFGASVEALLALPLTWCLLALSLIDADHQLLPDVLVL
PTMWLGLIVNAFGIHVPLADALWGAVAGYLSLWTVYWVFRLVTGKEGMGYGDFKLMALIGAWGGWQVLPLTLLLSSVVGA
LVGLCLLRFRRHALGTAIPFGPYLAIAGWIAVLWGDEIYASYIQLLGY

Nucleotide

• Length: 867 bp

>NTDB_id=412563 GR140_RS03385 WP_003247536.1 745096..745962(-) (pilD) [Pseudomonas putida strain YC-AE1]
ATGACTTTATGGGCTTTGCTGGCTGAACAGCCAGCGTATTTCCTCACCCTTGCCACGGTGCTCGGCCTGCTGGTGGGCAG
CTTTATCAACGTGCTGGTGTATCGCCTGCCGATCATGCTGGAGCGGCAATGGCAGCGTGAGGCGCAAGAGGTATTGGGGC
TGCCGACCACGCAGCACCCGCGTTTCGACCTGTGCCTGCCCGCCTCCCGGTGCCCACACTGCGCGCACCGGATTCGTGCG
TGGGAGAACATTCCGGTCATCAGCTACCTGGCCTTGGGCGGGCGCTGTTCGTCCTGCAAAAACCGCATCAGCCTGCGTTA
CCCGGTAGTGGAAGTGGCCAGCGCGCTGCTGTCGCTGGTAGTGGCCTGGCGCTTCGGCGCTTCGGTAGAGGCGTTGCTGG
CGTTGCCGCTGACCTGGTGCCTGCTGGCGCTGAGCCTGATCGATGCGGACCACCAGCTGCTGCCGGATGTGCTGGTGCTG
CCGACCATGTGGCTAGGGCTGATCGTCAATGCCTTTGGCATCCATGTGCCCCTGGCCGACGCACTGTGGGGCGCAGTGGC
CGGGTACCTCAGCCTGTGGACGGTGTACTGGGTGTTCAGGCTGGTCACCGGCAAGGAAGGCATGGGCTATGGCGATTTCA
AGCTGATGGCGCTGATCGGGGCGTGGGGGGGTTGGCAGGTGCTGCCGTTGACGCTGCTGTTGTCGTCGGTGGTGGGGGCG
CTTGTTGGCCTGTGCTTGCTGCGTTTTCGCCGGCATGCCTTGGGCACGGCTATCCCGTTTGGCCCTTATCTGGCAATTGC
GGGGTGGATTGCCGTGCTCTGGGGTGATGAAATATACGCCTCATACATACAACTGCTTGGATACTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.986	96.181	0.5
pilD	Acinetobacter baumannii D1279779	49.11	97.569	0.479
pilD	Acinetobacter nosocomialis M2	48.399	97.569	0.472
pilD	Vibrio campbellii strain DS40M4	49.091	95.486	0.469
pilD	Neisseria gonorrhoeae MS11	48.327	93.403	0.451