Detailed information NTG0412223

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: F3137_RS20665
• Genome accession: NZ_CP047283
• Coordinates: 4250599..4251399 (+)
• Length: 266 a.a.
• NCBI ID: WP_017901157.1
• Uniprot ID: -
• Organism: Klebsiella quasipneumoniae strain N18-04116
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4245599..4256399

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F3137_RS20630 (F3137_20445)	gspH	4245752..4246267 (+)	516	WP_004204416.1	type II secretion system minor pseudopilin GspH	-
F3137_RS20635 (F3137_20450)	gspI	4246264..4246629 (+)	366	WP_004204415.1	type II secretion system minor pseudopilin GspI	-
F3137_RS20640 (F3137_20455)	gspJ	4246626..4247222 (+)	597	WP_032426022.1	type II secretion system minor pseudopilin GspJ	-
F3137_RS20645 (F3137_20460)	gspK	4247219..4248199 (+)	981	WP_004204412.1	type II secretion system minor pseudopilin GspK	-
F3137_RS20650 (F3137_20465)	gspL	4248192..4249388 (+)	1197	WP_004204411.1	type II secretion system protein GspL	-
F3137_RS20655 (F3137_20470)	-	4249381..4249866 (+)	486	WP_004204409.1	type II secretion system protein M	-
F3137_RS20660 (F3137_20475)	-	4249856..4250584 (+)	729	WP_004204408.1	type II secretion system protein N	-
F3137_RS20665 (F3137_20480)	pilD	4250599..4251399 (+)	801	WP_017901157.1	A24 family peptidase	Machinery gene
F3137_RS20670 (F3137_20485)	hrpB	4251447..4253876 (-)	2430	WP_004204406.1	ATP-dependent helicase HrpB	-
F3137_RS20675 (F3137_20490)	thpR	4253949..4254485 (+)	537	WP_004204405.1	RNA 2',3'-cyclic phosphodiesterase	-
F3137_RS20680 (F3137_20495)	sfsA	4254485..4255201 (+)	717	WP_004204404.1	DNA/RNA nuclease SfsA	-
F3137_RS20685 (F3137_20500)	dksA	4255364..4255819 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29338.86 Da
• Isoelectric Point: 8.8894

>NTDB_id=412223 F3137_RS20665 WP_017901157.1 4250599..4251399(+) (pilD) [Klebsiella quasipneumoniae strain N18-04116]
MTTLAALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLAQTADDEPLTLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQARISWSYPLTELTTGLLFILAGALLAPGLALAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQALPQVLLFASASGLTWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGVFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=412223 F3137_RS20665 WP_017901157.1 4250599..4251399(+) (pilD) [Klebsiella quasipneumoniae strain N18-04116]
ATGACGACATTAGCCGCATTATCGCTGCATTTCCCCCTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTACCGTCTGCCGCGCATGCTGGCCCAGACCGCGGACGACGAGCCGCTAACCCTCAGCA
CGCCGGGCTCATCCTGTCCGCAGTGTCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGCTTCCTCTGGCTA
GGCCGTCGCGCCCGCTGCTGTCAGGCGCGCATCTCGTGGAGCTATCCGCTGACCGAGCTGACCACCGGCCTGCTGTTTAT
CCTCGCGGGGGCGCTGCTCGCACCGGGACTGGCGCTGGCGGGCGGCCTGGTGCTGCTCTCCTTTCTGCTGATACTGGCGC
GGATTGATGCCCGGACCCAGCTGCTGCCCGATCGCCTGACCCTGCCCCTGCTGTGGGCCGGACTGCTGTTTAATCTTAAC
GAGGTGTACATTGCGCTGCCGGACGCGGTCGCCGGGGCGATGGCTGGTTATCTGGCACTGTGGTCGGTCTATTGGCTGTT
CCGCCTGCTGACCGGCAAAGAGGCTCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGCTCTGCCGCAGGTGCTGTTGTTCGCCTCGGCCAGCGGGCTGACGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGAGGGGGCGTTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-230)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425