Detailed information NTG0412084

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: F3090_RS21730
• Genome accession: NZ_CP047271
• Coordinates: 4370939..4371739 (+)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain N16-03892
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4365939..4376739

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F3090_RS21695 (F3090_21330)	gspH	4366095..4366610 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
F3090_RS21700 (F3090_21335)	gspI	4366607..4366972 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
F3090_RS21705 (F3090_21340)	gspJ	4366969..4367565 (+)	597	WP_023279845.1	type II secretion system minor pseudopilin GspJ	-
F3090_RS21710 (F3090_21345)	gspK	4367562..4368548 (+)	987	WP_021467522.1	type II secretion system minor pseudopilin GspK	-
F3090_RS21715 (F3090_21350)	gspL	4368535..4369728 (+)	1194	WP_048979736.1	type II secretion system protein GspL	-
F3090_RS21720 (F3090_21355)	-	4369721..4370206 (+)	486	WP_004151943.1	type II secretion system protein M	-
F3090_RS21725 (F3090_21360)	-	4370196..4370942 (+)	747	WP_004145897.1	type II secretion system protein N	-
F3090_RS21730 (F3090_21365)	pilD	4370939..4371739 (+)	801	WP_004213441.1	prepilin peptidase	Machinery gene
F3090_RS21735 (F3090_21370)	hrpB	4371787..4374216 (-)	2430	WP_040225255.1	ATP-dependent helicase HrpB	-
F3090_RS21740 (F3090_21375)	thpR	4374289..4374825 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
F3090_RS21745 (F3090_21380)	sfsA	4374825..4375541 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
F3090_RS21750 (F3090_21385)	dksA	4375704..4376159 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=412084 F3090_RS21730 WP_004213441.1 4370939..4371739(+) (pilD) [Klebsiella pneumoniae strain N16-03892]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=412084 F3090_RS21730 WP_004213441.1 4370939..4371739(+) (pilD) [Klebsiella pneumoniae strain N16-03892]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425