Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   GKS41_RS08180 Genome accession   NZ_CP047268
Coordinates   1604578..1606407 (+) Length   609 a.a.
NCBI ID   WP_094296111.1    Uniprot ID   A0AAP3YFQ4
Organism   Bacillus velezensis strain DH8043     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1599578..1611407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKS41_RS08155 - 1600240..1600818 (+) 579 WP_007409110.1 GNAT family N-acetyltransferase -
  GKS41_RS08160 spx 1600997..1601392 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  GKS41_RS08165 - 1601450..1602106 (-) 657 WP_014417421.1 TerC family protein -
  GKS41_RS08170 mecA 1602382..1603038 (+) 657 WP_003155032.1 adaptor protein MecA Regulator
  GKS41_RS08175 - 1603190..1604350 (+) 1161 WP_159354008.1 competence protein CoiA family protein -
  GKS41_RS08180 pepF 1604578..1606407 (+) 1830 WP_094296111.1 oligoendopeptidase F Regulator
  GKS41_RS19745 - 1606445..1606612 (-) 168 WP_003155026.1 hypothetical protein -
  GKS41_RS08185 spxH 1606898..1607800 (-) 903 WP_003155024.1 protease adaptor protein SpxH -
  GKS41_RS08190 - 1607797..1608195 (-) 399 WP_003155023.1 thiol management oxidoreductase -
  GKS41_RS08195 - 1608424..1609110 (-) 687 WP_159354009.1 lytic transglycosylase domain-containing protein -
  GKS41_RS08200 - 1609115..1609687 (-) 573 WP_014417426.1 CYTH domain-containing protein -
  GKS41_RS08205 - 1609812..1610177 (+) 366 WP_003155020.1 hypothetical protein -
  GKS41_RS08210 yjbM 1610205..1610840 (+) 636 WP_007610625.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69798.48 Da        Isoelectric Point: 5.3508

>NTDB_id=412002 GKS41_RS08180 WP_094296111.1 1604578..1606407(+) (pepF) [Bacillus velezensis strain DH8043]
MTKENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=412002 GKS41_RS08180 WP_094296111.1 1604578..1606407(+) (pepF) [Bacillus velezensis strain DH8043]
ATGACTAAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATCCCGAATTTATCTTCTTTTAAAGGCA
CACTTGCTCATTCGGCTGATAATTTATACGCGGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGGAAACTTTAT
GCATACGCGCATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCTCAGGCTGCAAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTTATTC
TCGAAAAAGAAGAGCTTAAGCTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCGTCGCCTTCCAATACATTCAGCGTTTTAAATAACGCCGATAT
TACATTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTTCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCTGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTGAAAAAAGATAATTTTTACGCCCGTGTGAAAAAATATAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATCCCTGAAGAAGTTTACGATAACCTGATTAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTGCTCGGCCTTGATGAAGTCCATATTTATGACCTGTACACACCGCTCGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGATTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTGATGAACTGGCATGATAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCATACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCAAC
GACTAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGGTTCAGAGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTAACGCCTGAACTGCTGAACGAACTTTATTATGATTTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCAACAG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAGCTGAACGAAATGGAAGAGCTTCTTTCGAAAGTGAACCCTTCGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50

97.537

0.488

Multiple sequence alignment