Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GSQ19_RS07065 Genome accession   NZ_CP047242
Coordinates   1780690..1782702 (-) Length   670 a.a.
NCBI ID   WP_011317260.1    Uniprot ID   -
Organism   Trichormus variabilis 0441     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1775690..1787702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSQ19_RS07040 (GSQ19_07040) - 1776269..1776469 (-) 201 WP_153228360.1 hypothetical protein -
  GSQ19_RS07045 (GSQ19_07045) - 1776527..1777531 (+) 1005 WP_011317256.1 hypothetical protein -
  GSQ19_RS07050 (GSQ19_07050) - 1777599..1778153 (-) 555 WP_011317257.1 hypothetical protein -
  GSQ19_RS07055 (GSQ19_07055) - 1778199..1779422 (-) 1224 WP_011317258.1 type II secretion system F family protein -
  GSQ19_RS07060 (GSQ19_07060) pilT 1779524..1780642 (-) 1119 WP_011317259.1 type IV pilus twitching motility protein PilT Machinery gene
  GSQ19_RS07065 (GSQ19_07065) pilB/pilB1 1780690..1782702 (-) 2013 WP_011317260.1 GspE/PulE family protein Machinery gene
  GSQ19_RS07070 (GSQ19_07070) grpE 1783110..1783856 (+) 747 WP_011317261.1 nucleotide exchange factor GrpE -
  GSQ19_RS07075 (GSQ19_07075) dnaK 1784132..1786093 (+) 1962 WP_011317262.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74978.89 Da        Isoelectric Point: 5.1833

>NTDB_id=411814 GSQ19_RS07065 WP_011317260.1 1780690..1782702(-) (pilB/pilB1) [Trichormus variabilis 0441]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYINTEQMRQALIESRKSGRPLTEVLESITGRQLSPEYLRQYKKQQLFELK
ILYGVEYLDPEVNDIGSTMVGNLIETLIPVDICRRHRLVPLSKNDEHTPPYVLVAMVAPDNLEASDDLNRILRPQGLTLQ
RMVITQEDYQRLINQYLDELAVRQKHLEQEKFTDINQDLENLGNLDLEDAPEEMEADLGSAMKGAEDAPVINLVNRILAK
ALHDKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLQIVQDMVSKPFGLILVTGPTGSGKTTSLYSALAEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCGECRIAYTPTTEELARYGMTASQEVSVTFYKANTIPAEAIAEAKAKNQLCPACNG
VGYKGRCGVYEVMRVTENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKR
KTGLTCRTCSAILQPEWLDCPYCLTARFED

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=411814 GSQ19_RS07065 WP_011317260.1 1780690..1782702(-) (pilB/pilB1) [Trichormus variabilis 0441]
ATGACTTACTCGTCACCACAACGGCGCAGTACCGCTCTTACTACAAGAACAGAGTTTTCACCTTTCGGTAATAAGCTAGT
GCAGTCTGGCTACATTAACACCGAACAGATGAGACAGGCGCTGATTGAAAGTCGCAAGTCTGGTAGACCCTTAACAGAAG
TTTTGGAGTCAATTACTGGGCGACAATTATCACCTGAGTACCTCAGGCAGTACAAGAAACAACAATTATTTGAACTGAAA
ATACTATACGGTGTTGAATACCTCGATCCGGAGGTCAACGATATCGGTAGCACGATGGTGGGTAATTTAATTGAAACCCT
CATCCCAGTTGATATCTGCCGTCGTCATCGTTTAGTACCACTATCTAAAAATGATGAACACACCCCGCCTTATGTTTTAG
TGGCGATGGTTGCTCCGGATAACTTGGAAGCGTCGGACGACCTAAACCGCATCTTGCGCCCCCAAGGTTTAACATTACAG
CGTATGGTAATTACCCAGGAGGATTACCAACGGCTAATTAACCAATATTTGGATGAATTGGCTGTGCGGCAAAAGCACCT
GGAACAAGAAAAATTTACAGATATTAATCAGGATTTAGAAAATCTTGGCAATCTCGACCTAGAAGATGCTCCTGAAGAGA
TGGAGGCTGATTTAGGTTCAGCGATGAAGGGTGCAGAAGATGCACCTGTCATCAACCTCGTTAACAGAATTTTAGCGAAG
GCGTTGCATGACAAGGTTTCTGATATCCACATTGAACCCCAAGAAGAAAATCTGCGGATTCGCTTCCGCAAGGATGGGGT
ACTGCGGGAGGCTTTTGACCCCTTACCGAAAAAAATTATTCCGGCTGTAACAGCCAGATTTAAAATTATCTCCAATTTGG
ATATTGCCGAGCGACGTTTACCGCAAGATGGACGCATCCGCCGGATGTTTGAGGGACGTAAAGTAGACTTCCGCGTGAAT
ACCTTACCCAGTCGCTATGGGGAAAAGGTAGTACTGCGGATTTTGGACAACTCTTCTACGCAACTGGGACTGAATAAGTT
AATTACTGACCCAGAAACATTGCAGATTGTCCAAGATATGGTGAGTAAGCCTTTCGGTTTAATTTTGGTAACTGGGCCAA
CTGGTTCAGGTAAGACAACTTCCTTATATTCAGCACTGGCGGAGAAGAACTCACCAGGTATTAATATCAGTACGGTAGAA
GACCCAATTGAGTACAGTTTGCCAGGGATTACCCAGGTACAGGTAATTAGAGAAAAAGGGCTAGATTTTGCGACGGCATT
ACGGGCTTTTTTGCGACAAGACCCCGATGTGTTGCTGGTGGGTGAAACAAGAGACAAAGAAACAGCGAAAACAGCGATTG
AAGCGGCTTTAACAGGTCACTTGGTATTAACTACATTACACACCAACGATGCCCCTGGTGCGATCGCTCGTTTGGGTGAA
ATGGGTATTGAACCATTCATGGTTTCCAGTTCTCTGATTGGGGTATTGGCACAACGTTTAGTGCGCCGTGTTTGTGGTGA
GTGCCGTATTGCTTACACTCCCACCACTGAGGAGTTAGCTCGTTATGGCATGACGGCTTCTCAAGAAGTGTCTGTCACCT
TCTATAAAGCTAACACCATCCCCGCAGAAGCGATCGCCGAGGCCAAAGCGAAAAACCAGCTTTGTCCAGCTTGTAACGGT
GTTGGCTACAAAGGACGCTGTGGTGTTTATGAAGTGATGCGGGTTACAGAGAACCTACAAACTTTAATCAACGAAGAAGC
ACCAACAGAACGCATCAAAGAAGTAGCTGTAGAAGAAGGGATGAAAACCTTATTAGCTTATAGCTTAGACCTCGTTCGCC
AAGGTGCTACCACCCTAGAAGAAGTGGAACGGGTGACATTTACAGACACAGGTTTAGAAGCTGAATTAAAAGCTAAACGC
AAGACTGGTCTTACCTGCCGGACTTGCAGCGCCATATTACAACCAGAATGGCTAGATTGTCCCTACTGTCTAACAGCCCG
TTTTGAAGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-179)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.881

100

0.651
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.11

94.776

0.361

Multiple sequence alignment