Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   H5C78_RS19605 Genome accession   NZ_CP059917
Coordinates   4088049..4088708 (+) Length   219 a.a.
NCBI ID   WP_001221478.1    Uniprot ID   B7UIT0
Organism   Escherichia coli strain 64.1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4083049..4093708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5C78_RS19590 (H5C78_19590) ygiS 4085254..4086861 (-) 1608 WP_000288665.1 ABC transporter substrate-binding protein -
  H5C78_RS19595 (H5C78_19595) ygiV 4086970..4087452 (-) 483 WP_000183493.1 GyrI-like domain-containing protein -
  H5C78_RS19600 (H5C78_19600) ygiW 4087505..4087897 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  H5C78_RS19605 (H5C78_19605) ciaR 4088049..4088708 (+) 660 WP_001221478.1 quorum sensing response regulator transcription factor QseB Regulator
  H5C78_RS19610 (H5C78_19610) qseC 4088705..4090054 (+) 1350 WP_000673349.1 quorum sensing histidine kinase QseC -
  H5C78_RS19615 (H5C78_19615) mdaB 4090164..4090745 (+) 582 WP_000065421.1 NADPH:quinone oxidoreductase MdaB -
  H5C78_RS19620 (H5C78_19620) ygiN 4090776..4091090 (+) 315 WP_000633738.1 putative quinol monooxygenase -
  H5C78_RS19625 (H5C78_19625) - 4091135..4092022 (-) 888 WP_000614959.1 MurR/RpiR family transcriptional regulator -
  H5C78_RS19630 (H5C78_19630) - 4092019..4092966 (-) 948 WP_001318046.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24701.65 Da        Isoelectric Point: 6.9850

>NTDB_id=411566 H5C78_RS19605 WP_001221478.1 4088049..4088708(+) (ciaR) [Escherichia coli strain 64.1]
MRILLIEDDMLIGDGIKTGLSKMGFSIDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=411566 H5C78_RS19605 WP_001221478.1 4088049..4088708(+) (ciaR) [Escherichia coli strain 64.1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCAT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UIT0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment