Detailed information NTG0411440

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GS419_RS22380
• Genome accession: NZ_CP047192
• Coordinates: 4554353..4555153 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain Kp36
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4549353..4560153

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GS419_RS22345 (GS419_22345)	gspH	4549509..4550024 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
GS419_RS22350 (GS419_22350)	gspI	4550021..4550386 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
GS419_RS22355 (GS419_22355)	gspJ	4550383..4550979 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
GS419_RS22360 (GS419_22360)	gspK	4550976..4551962 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
GS419_RS22365 (GS419_22365)	gspL	4551949..4553142 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
GS419_RS22370 (GS419_22370)	-	4553135..4553620 (+)	486	WP_004151943.1	type II secretion system protein M	-
GS419_RS22375 (GS419_22375)	-	4553610..4554356 (+)	747	WP_004145897.1	type II secretion system protein N	-
GS419_RS22380 (GS419_22380)	pilD	4554353..4555153 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
GS419_RS22385 (GS419_22385)	hrpB	4555201..4557630 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
GS419_RS22390 (GS419_22390)	thpR	4557703..4558239 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
GS419_RS22395 (GS419_22395)	sfsA	4558239..4558955 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
GS419_RS22400 (GS419_22400)	dksA	4559118..4559573 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=411440 GS419_RS22380 WP_002888947.1 4554353..4555153(+) (pilD) [Klebsiella pneumoniae strain Kp36]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=411440 GS419_RS22380 WP_002888947.1 4554353..4555153(+) (pilD) [Klebsiella pneumoniae strain Kp36]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425