Detailed information NTG0411052

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GST84_RS03355
• Genome accession: NZ_CP047152
• Coordinates: 715273..716139 (-)
• Length: 288 a.a.
• NCBI ID: WP_135192881.1
• Uniprot ID: -
• Organism: Pseudomonas putida strain PP27
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 710273..721139

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GST84_RS03320 (GST84_03285)	-	710662..711957 (-)	1296	WP_060546218.1	NAD(P)/FAD-dependent oxidoreductase	-
GST84_RS03325 (GST84_03290)	-	712042..712215 (-)	174	WP_008091951.1	DUF3094 domain-containing protein	-
GST84_RS03330 (GST84_03295)	-	712348..712977 (-)	630	WP_003247546.1	DUF1780 domain-containing protein	-
GST84_RS03335 (GST84_03300)	-	713033..713464 (-)	432	WP_103443441.1	hypothetical protein	-
GST84_RS03340 (GST84_03305)	-	713552..714259 (+)	708	WP_103470188.1	energy-coupling factor ABC transporter permease	-
GST84_RS03345 (GST84_03310)	yacG	714453..714653 (-)	201	WP_010951889.1	DNA gyrase inhibitor YacG	-
GST84_RS03350 (GST84_03315)	coaE	714650..715273 (-)	624	WP_004575721.1	dephospho-CoA kinase	-
GST84_RS03355 (GST84_03320)	pilD	715273..716139 (-)	867	WP_135192881.1	A24 family peptidase	Machinery gene
GST84_RS03360 (GST84_03325)	-	716142..717350 (-)	1209	WP_135192882.1	type II secretion system F family protein	-
GST84_RS03365 (GST84_03330)	-	717567..717977 (+)	411	WP_012051131.1	pilin	-
GST84_RS03375 (GST84_03340)	-	718425..719575 (+)	1151	WP_087535084.1	IS3 family transposase	-
GST84_RS03380 (GST84_03345)	-	719614..720003 (-)	390	WP_135192645.1	tautomerase family protein	-
GST84_RS03385 (GST84_03350)	-	720106..720948 (+)	843	WP_135192644.1	LysR family transcriptional regulator	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31874.92 Da
• Isoelectric Point: 7.5986

>NTDB_id=411052 GST84_RS03355 WP_135192881.1 715273..716139(-) (pilD) [Pseudomonas putida strain PP27]
MTLWALLAEQPAYFLTLATVLGLLVGSFINVLVYRLPIMLERQWQREAQEVLGLPTTQHPRFDLCLPASRCPHCAHRIRA
WENIPVISYLALGGRCSSCKNRISLRYPVVEVASALLSLVVAWRFGASVEALLALPLTWCLLALSLIDADHQLLPDVLVL
PTMWLGLIVNAFGIHVALADALWGAVAGYLSLWTVYWVFRLVTGKEGMGYGDFKLMALIGAWGGWQVLPLTLLLSSVVGA
LFGLCLLRFRRDAMGTAIPFGPYLAIAGWIAVLWGDEIYTSYMQLLGY

Nucleotide

• Length: 867 bp

>NTDB_id=411052 GST84_RS03355 WP_135192881.1 715273..716139(-) (pilD) [Pseudomonas putida strain PP27]
ATGACTTTATGGGCTTTGCTGGCTGAACAGCCAGCGTATTTCCTCACCCTTGCCACGGTGCTCGGCCTGCTGGTGGGCAG
CTTTATCAACGTGCTGGTGTATCGCCTGCCGATCATGCTGGAGCGCCAATGGCAGCGTGAGGCGCAAGAGGTATTGGGGC
TGCCGACCACGCAGCACCCGCGTTTCGACCTGTGCCTGCCCGCCTCCCGGTGCCCACACTGCGCGCACCGGATTCGTGCG
TGGGAAAACATTCCGGTCATCAGCTACCTGGCCTTGGGCGGGCGCTGTTCGTCCTGCAAAAACCGCATCAGCCTGCGTTA
CCCGGTAGTGGAAGTGGCCAGCGCGCTGCTGTCGCTGGTAGTGGCCTGGCGCTTCGGCGCTTCGGTAGAGGCGTTGCTGG
CGTTGCCGCTGACCTGGTGCCTGCTGGCGCTGAGCCTGATCGATGCGGACCACCAGTTGCTGCCGGATGTGCTGGTGCTG
CCGACCATGTGGCTAGGGCTGATCGTCAATGCCTTTGGTATCCATGTGGCCCTGGCCGACGCACTGTGGGGCGCAGTGGC
CGGGTACCTCAGCCTGTGGACGGTGTACTGGGTGTTCAGGCTGGTCACCGGCAAGGAAGGCATGGGCTATGGCGATTTCA
AGCTGATGGCGCTGATCGGGGCGTGGGGGGGTTGGCAGGTGCTGCCGTTGACGCTGCTGTTGTCGTCGGTGGTGGGGGCG
CTGTTTGGCCTGTGCCTGCTGCGTTTTCGCCGGGATGCCATGGGCACGGCAATCCCGTTTGGCCCTTATCTGGCAATTGC
GGGGTGGATTGCCGTGCTCTGGGGTGATGAAATATACACCTCATACATGCAACTGCTTGGATACTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.347	96.181	0.503
pilD	Acinetobacter baumannii D1279779	49.822	97.569	0.486
pilD	Acinetobacter nosocomialis M2	49.11	97.569	0.479
pilD	Vibrio campbellii strain DS40M4	49.275	95.833	0.472
pilD	Neisseria gonorrhoeae MS11	47.955	93.403	0.448