Detailed information NTG0410153

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GRB21_RS05010
• Genome accession: NZ_CP047073
• Coordinates: 1003197..1004069 (-)
• Length: 290 a.a.
• NCBI ID: WP_005770019.1
• Uniprot ID: Q888U2
• Organism: Pseudomonas syringae pv. tomato strain delta X
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 998197..1009069

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GRB21_RS04970	-	998907..1000205 (-)	1299	WP_005614782.1	NAD(P)/FAD-dependent oxidoreductase	-
GRB21_RS04980	-	1000422..1000955 (-)	534	WP_005770014.1	MOSC domain-containing protein	-
GRB21_RS04985	-	1000952..1001581 (-)	630	WP_003381213.1	DUF1780 domain-containing protein	-
GRB21_RS04995	-	1001778..1002104 (+)	327	WP_005770015.1	hypothetical protein	-
GRB21_RS05000	yacG	1002371..1002580 (-)	210	WP_003381207.1	DNA gyrase inhibitor YacG	-
GRB21_RS05005	coaE	1002577..1003200 (-)	624	WP_005770017.1	dephospho-CoA kinase	-
GRB21_RS05010	pilD	1003197..1004069 (-)	873	WP_005770019.1	prepilin peptidase	Machinery gene
GRB21_RS05015	pilC	1004071..1005288 (-)	1218	WP_011103349.1	type II secretion system F family protein	Machinery gene
GRB21_RS05020	pilB	1005291..1006985 (-)	1695	WP_011103350.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GRB21_RS05025	-	1007210..1007638 (+)	429	WP_011103351.1	pilin	-
GRB21_RS05030	-	1007834..1008202 (-)	369	WP_011103352.1	winged helix-turn-helix transcriptional regulator	-
GRB21_RS05035	gstA	1008360..1008980 (+)	621	WP_011103353.1	glutathione transferase GstA	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31982.22 Da
• Isoelectric Point: 8.1553

>NTDB_id=410153 GRB21_RS05010 WP_005770019.1 1003197..1004069(-) (pilD) [Pseudomonas syringae pv. tomato strain delta X]
MPLLDLLASSPLAFVTTCCLLGLIVGSFLNVVVYRLPKMMERDWKVQSREMLGLPAEPDQPVFNLILPHSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPVVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLSDALWGAVAGYLTLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=410153 GRB21_RS05010 WP_005770019.1 1003197..1004069(-) (pilD) [Pseudomonas syringae pv. tomato strain delta X]
ATGCCCCTCCTCGACCTCTTGGCCAGCTCTCCCCTGGCCTTCGTCACCACCTGCTGCCTACTTGGCCTGATCGTCGGAAG
CTTCCTCAACGTGGTCGTCTACCGCCTCCCAAAAATGATGGAGCGCGACTGGAAAGTACAATCACGGGAAATGCTAGGCC
TCCCTGCCGAACCGGACCAACCCGTCTTCAACCTGATCCTGCCGCACTCAAGCTGCCCGCACTGCGCGCACAAGATCCGC
CCGTGGGAAAACCTGCCGGTCATCAGTTACCTGTTGCTGCGCGGCAAATGCTCGCAATGCAAAGCCCCCATCAGCAAGCG
CTACCCAGTGGTGGAGCTGACCTGCGCAGTGTTGTCGGCCTACGTCGCCTGGCATTTCGGCTTCGGCTGGCAGGCGGCTG
CGATGCTGGTGTTGAGCTGGGGTTTGCTGGCGATGAGTCTGATCGATGCCGATCATCAGCTGTTACCTGACTCTTTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTTAACGCCTTTGGGCTGTTCACCTCGCTGAGCGACGCGCTGTGGGGTGCAGT
GGCCGGTTATCTGACGCTGTGGTCGGTGTTCTGGCTTTTCAAGTTGGTGACGGGCAAGGAAGGCATGGGCTATGGCGACT
TCAAATTGCTGGCCATGCTTGGCGCGTGGGGTGGCTGGCAGATTCTGCCGTTGACCATTCTGTTGTCGTCGCTGGTGGGT
GCGGTACTTGGGGTGATCATGATGCGCGTGCGCAGGGTCGAAAGTGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTATATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: Q888U2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.064	97.586	0.528
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.555	94.483	0.497
pilD	Acinetobacter nosocomialis M2	51.825	94.483	0.49
pilD	Neisseria gonorrhoeae MS11	51.866	92.414	0.479