Detailed information NTG0409769

Bioinformatically predicted

Overview

• Name: pilJ
• Type: Machinery gene
• Locus tag: GQR42_RS14025
• Genome accession: NZ_CP046973
• Coordinates: 2758172..2760835 (+)
• Length: 887 a.a.
• NCBI ID: WP_158200432.1
• Uniprot ID: A0A857D4J8
• Organism: Microcystis aeruginosa FD4
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2753172..2765835

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GQR42_RS14005 (GQR42_14035)	-	2753470..2753694 (-)	225	WP_158200428.1	hypothetical protein	-
GQR42_RS29545	-	2753821..2753949 (-)	129	WP_257792579.1	hypothetical protein	-
GQR42_RS14010 (GQR42_14040)	hmpF	2754086..2755726 (-)	1641	WP_158200429.1	pilus motility taxis protein HmpF	-
GQR42_RS14015 (GQR42_14045)	pilH	2757091..2757456 (+)	366	WP_158200430.1	response regulator transcription factor	Machinery gene
GQR42_RS14020 (GQR42_14050)	-	2757548..2758078 (+)	531	WP_158200431.1	chemotaxis protein CheW	-
GQR42_RS14025 (GQR42_14055)	pilJ	2758172..2760835 (+)	2664	WP_158200432.1	methyl-accepting chemotaxis protein	Machinery gene
GQR42_RS14030 (GQR42_14060)	-	2760905..2762086 (-)	1182	WP_158200433.1	RNA-guided endonuclease TnpB family protein	-
GQR42_RS14035 (GQR42_14065)	-	2762284..2763495 (+)	1212	WP_158200434.1	glycosyltransferase	-
GQR42_RS14040 (GQR42_14070)	-	2763942..2765279 (+)	1338	WP_158200435.1	AAA-like domain-containing protein	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 96379.25 Da
• Isoelectric Point: 4.4932

>NTDB_id=409769 GQR42_RS14025 WP_158200432.1 2758172..2760835(+) (pilJ) [Microcystis aeruginosa FD4]
MPSGTEYAKLYGQANIAYCQGNLENAAVIIKDMIGKYPDDPNVLLLQGHIDLGLQKYSLARESYEKVLQLAKNSPNRSDL
VDYARQGLDQIRQWRFKAEDEDFTIAATESIAYREAAFDPGQGFSNSRSRESQAADPDWQNEGMDSDSGLFNQEALSEPR
VGKVDSYDDDFGESEETAFSSLTLSSSGSHGSQWSGDSAKGEPAFPFVEAAEIDSLQEGWGGELGSTGEAPFMVSSDRRE
NPLTGRDQELSSTDTLGESNFAIDLEPTQRQGAKTGLNQGRVPQKRYEEDDARDFREPKLNDEDLDGFPPLALTDLARGL
GDSDLFTRSGGEALSSGFAPEASGSLNASSGVGEPNFNPSRLKIPDDKRDSHLANASDRLLKPVVEVNPGKLAFFVNASL
GKKQLILAALAGITPVILIFAVSNASWISNLLTAKPAPNSKEKTEKVAPKPAPASLSPFSQPKPAMMLLTGVGTFALTWL
GLQLLISQIKRSLNDLQSQFAHLSEGNFNAKATIYSEDEFGQLANRFNQMARVVVTTTREAQGRAAETEREREDLQRQVI
RLLDDVEGAARGDLTVEAEVSADVLGAVADAFNLTIQNLREIVRQVKKAAKQVNQASTNSESFARNQSSDALRMAEELAV
TLNSVQMMTDSIQRVAENAREAEDVARTSSITALKGGDSVERTVVGILQIRDTVSETARKVKRLAVASQEISKIVAVISQ
IASRTNLLALNASIQAARAGDAGRGFAVVADEVRQLADRSAKSLKEIEQIVLQIQSETGSVMTAMEEGIQQVIDVTERSE
QAKKSLEDIIQVSNRIDTLVRSITADTVKQRENSLNVAQVMQSVELTAQETSQESQRVAGSLQKLVSISRELLSSVERFK
VDSEDDQ

Nucleotide

• Length: 2664 bp

>NTDB_id=409769 GQR42_RS14025 WP_158200432.1 2758172..2760835(+) (pilJ) [Microcystis aeruginosa FD4]
ATGCCGTCAGGGACAGAATACGCAAAACTCTATGGACAGGCTAACATCGCCTATTGCCAGGGCAACCTTGAGAATGCTGC
CGTCATTATCAAAGACATGATCGGCAAGTATCCCGATGATCCCAACGTGCTGCTTTTACAGGGCCACATTGATCTAGGAC
TACAAAAATACAGCTTGGCTAGGGAAAGTTACGAAAAAGTGCTGCAATTGGCTAAAAATTCCCCCAATCGCTCCGATCTG
GTGGATTATGCTCGACAGGGGCTCGATCAGATTCGGCAATGGCGTTTTAAAGCCGAGGATGAAGATTTTACGATCGCTGC
TACGGAAAGCATTGCCTACAGGGAGGCAGCTTTCGATCCAGGTCAAGGTTTTAGCAATTCTCGGTCGAGGGAGTCGCAAG
CGGCTGACCCCGATTGGCAAAATGAAGGGATGGATTCGGATAGTGGTCTTTTCAATCAGGAGGCTCTCAGCGAGCCTAGG
GTGGGTAAAGTCGATTCCTATGACGATGATTTTGGGGAATCGGAGGAAACCGCCTTTAGTTCCCTTACCCTGTCCAGTTC
AGGCTCTCACGGTTCCCAGTGGTCTGGCGATTCTGCAAAAGGGGAACCAGCTTTCCCCTTTGTAGAAGCAGCGGAGATTG
ACTCGCTGCAGGAAGGGTGGGGCGGTGAGTTAGGGTCAACGGGAGAAGCCCCTTTTATGGTTTCCTCTGACCGGCGGGAA
AATCCCCTCACTGGTAGAGATCAGGAGTTATCCTCTACCGATACCCTAGGAGAAAGCAATTTTGCCATTGACCTGGAACC
GACCCAGCGACAAGGGGCAAAAACTGGCCTTAATCAGGGCAGAGTCCCTCAAAAAAGATACGAGGAGGACGACGCCAGAG
ATTTTAGGGAACCAAAACTCAATGATGAGGATTTAGACGGTTTTCCCCCACTGGCTCTGACGGATCTGGCCCGAGGACTG
GGGGACTCAGATCTATTTACCCGTTCGGGAGGGGAGGCATTAAGCAGTGGTTTTGCCCCCGAAGCCAGTGGGTCCCTTAA
TGCCAGTTCTGGTGTGGGAGAGCCTAATTTTAATCCTTCTCGTCTAAAAATTCCCGATGACAAAAGAGATAGTCATCTTG
CCAATGCTTCCGATCGCTTGCTCAAGCCAGTGGTGGAAGTCAATCCGGGCAAATTAGCCTTTTTTGTCAATGCTTCTTTA
GGCAAAAAGCAACTAATTTTAGCGGCTTTAGCGGGTATTACCCCTGTAATCTTAATTTTTGCCGTTAGTAACGCCTCTTG
GATTTCTAACCTCTTGACTGCCAAACCAGCCCCGAATAGCAAGGAAAAAACCGAAAAAGTTGCCCCCAAACCAGCCCCAG
CCAGCCTTTCCCCTTTTAGTCAACCGAAACCAGCAATGATGTTATTAACTGGAGTGGGAACCTTTGCCCTGACTTGGCTA
GGTTTACAGTTGCTAATTAGTCAGATTAAGCGGAGTCTCAACGATCTGCAAAGCCAATTCGCTCACCTCTCGGAAGGTAA
TTTTAACGCTAAAGCGACGATCTACTCGGAAGATGAATTCGGTCAACTGGCCAATCGCTTCAATCAGATGGCCCGGGTGG
TGGTAACGACCACCCGGGAAGCCCAAGGCCGCGCCGCCGAAACGGAACGGGAACGGGAAGACCTACAACGGCAAGTGATT
CGACTTCTCGATGATGTGGAAGGAGCCGCTAGGGGGGATCTCACCGTGGAAGCGGAGGTAAGCGCCGATGTTTTAGGGGC
GGTGGCCGATGCTTTTAATTTGACTATCCAAAACTTGCGCGAAATTGTCCGACAGGTGAAAAAAGCGGCAAAACAGGTGA
ATCAAGCCAGTACCAATAGTGAATCTTTCGCCCGCAATCAGTCTAGCGATGCCCTGCGGATGGCGGAGGAATTGGCCGTC
ACCCTTAATTCGGTGCAGATGATGACCGACTCAATTCAACGGGTGGCAGAAAACGCCAGAGAAGCCGAAGATGTCGCCCG
CACTTCCTCGATTACTGCCCTGAAGGGGGGCGATTCCGTGGAAAGAACCGTGGTGGGAATTCTGCAAATCCGTGACACGG
TATCAGAAACGGCGCGAAAAGTCAAGCGTTTGGCGGTAGCTTCCCAGGAAATCTCGAAAATTGTTGCGGTAATCTCTCAG
ATTGCCTCGCGGACTAATTTATTGGCTCTTAATGCCTCGATTCAAGCGGCGCGAGCGGGAGATGCGGGCCGCGGTTTTGC
AGTTGTGGCCGATGAGGTGCGCCAGTTAGCCGATAGATCTGCTAAATCTCTTAAGGAAATTGAACAGATCGTTTTACAGA
TCCAATCGGAAACGGGTTCGGTAATGACGGCGATGGAGGAAGGGATTCAGCAGGTTATCGATGTCACCGAGCGCTCGGAA
CAGGCGAAGAAGTCCCTAGAAGATATTATCCAAGTTTCTAACCGCATCGATACGCTGGTGCGATCGATTACCGCCGATAC
GGTCAAACAACGGGAAAATTCCCTCAATGTCGCCCAAGTTATGCAGTCGGTGGAGTTAACCGCTCAGGAAACTTCCCAAG
AATCCCAACGGGTGGCGGGTTCTCTGCAAAAATTGGTGAGTATCTCCCGGGAGCTGCTGTCTTCGGTAGAACGGTTTAAG
GTAGACTCGGAGGATGATCAATAA

Domains

Predicted by InterproScan.

• Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal) (664-844)

• HAMP domain (HAMP) (484-532)

3D structure

• AlphaFold DB: A0A857D4J8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilJ	Synechocystis sp. PCC 6803	49.359	100	0.521