Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GPY14_RS15370 Genome accession   NZ_CP046918
Coordinates   3203250..3203687 (-) Length   145 a.a.
NCBI ID   WP_015416783.1    Uniprot ID   -
Organism   Bacillus velezensis strain BA-26     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3198250..3208687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY14_RS15345 (GPY14_15345) - 3199270..3200406 (+) 1137 WP_015416779.1 zinc-dependent alcohol dehydrogenase -
  GPY14_RS15350 (GPY14_15350) - 3200421..3200855 (+) 435 WP_033575151.1 RDD family protein -
  GPY14_RS15355 (GPY14_15355) - 3200972..3201250 (+) 279 WP_263612330.1 YckD family protein -
  GPY14_RS15360 (GPY14_15360) - 3201354..3202790 (+) 1437 WP_015416781.1 glycoside hydrolase family 1 protein -
  GPY14_RS15365 (GPY14_15365) nin/comJ 3202831..3203229 (-) 399 WP_015416782.1 competence protein ComJ Regulator
  GPY14_RS15370 (GPY14_15370) nucA/comI 3203250..3203687 (-) 438 WP_015416783.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GPY14_RS15375 (GPY14_15375) hxlB 3204045..3204602 (-) 558 WP_015416784.1 6-phospho-3-hexuloisomerase -
  GPY14_RS15380 (GPY14_15380) hxlA 3204599..3205234 (-) 636 WP_015416785.1 3-hexulose-6-phosphate synthase -
  GPY14_RS15385 (GPY14_15385) - 3205466..3205828 (+) 363 WP_014304320.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16038.34 Da        Isoelectric Point: 8.4850

>NTDB_id=409372 GPY14_RS15370 WP_015416783.1 3203250..3203687(-) (nucA/comI) [Bacillus velezensis strain BA-26]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLLKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=409372 GPY14_RS15370 WP_015416783.1 3203250..3203687(-) (nucA/comI) [Bacillus velezensis strain BA-26]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACATCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTGAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-132)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.016

86.897

0.634

Multiple sequence alignment