Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GO485_RS18670 Genome accession   NZ_CP046904
Coordinates   4357329..4358792 (+) Length   487 a.a.
NCBI ID   WP_145877940.1    Uniprot ID   A0A562PLU7
Organism   Pseudoduganella flava strain DSM 26639     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4352329..4363792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO485_RS18645 (GO485_18600) - 4353809..4354831 (+) 1023 WP_199271794.1 SIS domain-containing protein -
  GO485_RS18650 (GO485_18605) - 4354831..4355370 (+) 540 WP_145877949.1 HAD family hydrolase -
  GO485_RS18655 (GO485_18610) lptC 4355385..4355978 (+) 594 WP_145877948.1 LPS export ABC transporter periplasmic protein LptC -
  GO485_RS18660 (GO485_18615) lptA 4355996..4356565 (+) 570 WP_145877946.1 lipopolysaccharide transport periplasmic protein LptA -
  GO485_RS18665 (GO485_18620) lptB 4356567..4357322 (+) 756 WP_145877943.1 LPS export ABC transporter ATP-binding protein -
  GO485_RS18670 (GO485_18625) rpoN/rpoN1 4357329..4358792 (+) 1464 WP_145877940.1 RNA polymerase factor sigma-54 Machinery gene
  GO485_RS18675 (GO485_18630) hpf 4358877..4359233 (+) 357 WP_145877938.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  GO485_RS18680 (GO485_18635) - 4359423..4360505 (+) 1083 WP_145877936.1 enoyl-CoA hydratase/isomerase family protein -
  GO485_RS18685 (GO485_18640) - 4360513..4361505 (+) 993 WP_145877934.1 amidohydrolase family protein -
  GO485_RS18690 (GO485_18645) rapZ 4361575..4362438 (-) 864 WP_145877932.1 RNase adapter RapZ -
  GO485_RS18695 (GO485_18650) - 4362481..4363737 (-) 1257 WP_145877930.1 sodium:proton antiporter -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 54486.09 Da        Isoelectric Point: 5.2913

>NTDB_id=409256 GO485_RS18670 WP_145877940.1 4357329..4358792(+) (rpoN/rpoN1) [Pseudoduganella flava strain DSM 26639]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLTDNPLLERLDDPLDRSVRLLADGAINSTPATPEAPAEGP
PQEGAAPAEPEPYEGGEGDAPEGDHDWSDASRGKGPDDDDARPQLEASHGSLRDHLMEQMRVTVQEPRDRALMELIIDAL
DDNGYLEESLEEIHARLPEELEIELDELRTALKLLQNFDPVGVGARNASECLALQIRRMPHVPMVTRRMALCIVEKHLQW
FAQRDFNKLKKALDCDDEDLREAQAVIRQCNPHPGAAFARDVSDYVVPDVIVKKGRTGWIVTLNNDVMPRLRVNALYASL
LKQGKGEAQMNAQLQEAKWLIKNMRQRFDTILRVAQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHESTISRVTTQKY
MLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQFTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAKYREALKIPP
VSLRKSL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=409256 GO485_RS18670 WP_145877940.1 4357329..4358792(+) (rpoN/rpoN1) [Pseudoduganella flava strain DSM 26639]
ATGAAACAGTCTTTGCAACTGCGAACGTCGCAGCATCTCGCGCTGACCCCGCAGTTGCAGCAGTCGATCCGCCTGCTGCA
ATTGTCCACGCTGGAGCTGCACCAGGAACTGGAACAACTGCTGACGGATAACCCGCTGCTGGAGCGGCTCGACGATCCGC
TCGACCGTTCCGTGCGCCTGCTGGCCGACGGAGCAATCAACTCGACGCCCGCTACCCCGGAAGCGCCGGCCGAAGGGCCG
CCGCAGGAAGGCGCCGCGCCGGCCGAGCCGGAACCGTACGAGGGCGGCGAGGGCGATGCGCCCGAAGGCGATCACGACTG
GAGCGACGCCAGCCGCGGCAAGGGCCCGGACGACGACGACGCCCGCCCGCAGCTGGAAGCGAGCCACGGCAGCCTGCGCG
ACCATCTGATGGAACAGATGCGCGTCACGGTGCAGGAACCACGTGACCGTGCGCTGATGGAACTGATCATCGACGCGCTC
GACGACAACGGTTATCTCGAAGAGAGCCTGGAAGAGATCCACGCGCGCTTGCCGGAAGAGCTGGAGATCGAGCTCGACGA
GTTGCGCACGGCGCTGAAGCTGCTGCAGAATTTCGACCCAGTCGGCGTCGGCGCGCGCAACGCGTCCGAATGCCTGGCGC
TGCAGATCCGGCGCATGCCGCACGTGCCGATGGTGACGCGCCGCATGGCGCTGTGCATCGTCGAGAAACACCTGCAGTGG
TTCGCGCAGCGCGACTTCAACAAGCTGAAGAAAGCCCTCGATTGCGACGACGAAGACTTGCGCGAAGCGCAGGCCGTTAT
CCGCCAATGCAACCCGCACCCGGGCGCCGCGTTCGCGCGTGACGTGTCAGACTACGTGGTACCCGATGTGATCGTGAAGA
AAGGCCGCACCGGCTGGATTGTCACGCTGAACAACGACGTGATGCCGCGCCTGCGTGTGAATGCCCTGTACGCCAGCCTG
CTCAAGCAAGGCAAGGGCGAGGCGCAGATGAATGCTCAGCTGCAGGAAGCGAAGTGGCTGATCAAGAATATGCGTCAGCG
TTTCGACACGATCCTGCGCGTCGCCCAGGCCATCGTCGAGCGGCAGAAGAACTTTTTCTCGCATGGCGCTGTGGCAATGA
GACCCCTTGTGTTGCGCGAAATAGCTGATACACTGGGACTACACGAGAGCACTATCTCTCGGGTAACAACTCAAAAATAT
ATGCTGACCCCGCATGGCATGTTTGAGTTGAAGTACTTTTTTGGCAGCCATGTCGCAACCGAAGCTGGTGGGGAAGCTTC
CTCGACTGCGATCCGTGCGCTGATCGTGCAATTCACAGGAGCCGAAGACCCCAAGAACCCTTTATCCGACAGTAAGATTG
CGGACATGCTGGGTGAACAAGGCATGGTGATTGCGCGACGAACTGTTGCCAAATATCGCGAAGCATTGAAAATCCCCCCA
GTCAGCCTCCGCAAGTCCTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(330-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(126-317)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A562PLU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.99

100

0.632

Multiple sequence alignment