Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   GP482_RS00980 Genome accession   NZ_CP046875
Coordinates   192117..193415 (+) Length   432 a.a.
NCBI ID   WP_158913254.1    Uniprot ID   A0AAE6R4B7
Organism   Streptococcus ruminicola strain CNU_77-61     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 187117..198415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GP482_RS00955 (GP482_00955) - 187782..188426 (+) 645 WP_158913250.1 response regulator transcription factor -
  GP482_RS00960 (GP482_00960) - 188551..189951 (+) 1401 WP_158913252.1 Cof-type HAD-IIB family hydrolase -
  GP482_RS00965 (GP482_00965) - 189951..190307 (+) 357 WP_020916316.1 S1 RNA-binding domain-containing protein -
  GP482_RS00970 (GP482_00970) cysK 190407..191336 (-) 930 WP_074626743.1 cysteine synthase A -
  GP482_RS00975 (GP482_00975) - 191436..192062 (-) 627 WP_074603619.1 YigZ family protein -
  GP482_RS00980 (GP482_00980) comFA/cflA 192117..193415 (+) 1299 WP_158913254.1 DEAD/DEAH box helicase Machinery gene
  GP482_RS00985 (GP482_00985) - 193415..194077 (+) 663 WP_158913257.1 ComF family protein -
  GP482_RS00990 (GP482_00990) raiA 194154..194702 (+) 549 WP_074603611.1 ribosome-associated translation inhibitor RaiA -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 48942.24 Da        Isoelectric Point: 10.0425

>NTDB_id=409099 GP482_RS00980 WP_158913254.1 192117..193415(+) (comFA/cflA) [Streptococcus ruminicola strain CNU_77-61]
MTELNDYYGRIFTESQLTDDLKKKAKALPSVKKEKGQMICNRCGSVMAANQMLQTGRIYCRECLVLGRNTSDSNLYYFPQ
KPFTKTQSLKWQGQLTSYQDEVSKGMLKGISEKQDLLIHAVTGAGKTEMIYQSLAKVIDNGGSVCLASPRIDVCLELYKR
LSRDFSCPITLLHGDSEPYKRSPLVIATTHQLLKFYRAFDLLIIDEVDAFPFVDNKMLYHAVNQSLKDDGVKLFLTATST
DELDKKVKKGELTKLHLARRFHANPLVVPKMVWLSRVLISMKKKKLPPKLISLLKKQRQSQFPLLIFFPNIEQGREFTSI
LQTTFPDERIGFVSSKTENRLELVEEFRQGKLTILVTTTILERGVTFPCVDVFVLLANHKLYSKSALVQISGRVGRAAER
PTGELLFLHDGATKAMKKAISEIKEMNKKGGF

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=409099 GP482_RS00980 WP_158913254.1 192117..193415(+) (comFA/cflA) [Streptococcus ruminicola strain CNU_77-61]
ATGACAGAGTTGAATGATTACTATGGAAGAATTTTTACAGAAAGTCAATTAACAGATGACTTGAAAAAGAAAGCAAAAGC
CCTTCCTTCGGTTAAAAAGGAAAAAGGGCAAATGATTTGCAATCGCTGTGGAAGTGTAATGGCAGCCAATCAAATGTTGC
AAACTGGTCGCATTTATTGTAGAGAGTGTTTAGTTTTGGGGCGAAATACTAGTGATTCTAACCTTTATTATTTTCCTCAG
AAGCCTTTTACAAAGACTCAGTCGTTAAAGTGGCAAGGACAATTGACTTCTTATCAAGATGAAGTTTCTAAAGGCATGCT
TAAAGGCATTTCAGAAAAACAAGATTTATTGATTCACGCTGTGACTGGTGCTGGTAAAACTGAAATGATTTATCAATCTT
TAGCCAAAGTTATTGATAATGGAGGAAGTGTCTGCTTAGCAAGCCCAAGGATTGATGTTTGTTTAGAACTTTATAAACGG
TTATCCAGAGATTTTTCTTGTCCAATTACACTTTTGCATGGTGATTCTGAACCGTATAAACGCTCTCCGTTGGTAATTGC
AACGACCCATCAGCTTTTAAAATTTTACCGAGCTTTTGATTTATTAATCATCGACGAAGTTGATGCCTTTCCCTTTGTTG
ATAATAAAATGCTTTATCACGCGGTCAACCAATCCTTAAAAGATGATGGCGTTAAACTCTTTTTAACAGCGACTTCGACT
GATGAACTTGATAAAAAGGTGAAAAAAGGAGAGTTGACAAAATTGCATTTAGCAAGACGTTTTCATGCCAATCCTTTGGT
AGTCCCCAAAATGGTTTGGTTAAGCAGAGTGTTAATCTCAATGAAAAAGAAAAAACTTCCTCCTAAACTAATCTCTCTTT
TAAAAAAACAAAGGCAAAGTCAATTTCCGCTTCTTATCTTTTTTCCGAATATTGAACAAGGTAGAGAATTTACAAGTATC
TTACAAACCACTTTTCCAGATGAAAGGATAGGATTTGTATCAAGTAAAACTGAAAATCGTCTGGAACTGGTAGAAGAATT
TAGACAAGGGAAATTAACAATATTGGTGACAACAACCATCCTAGAACGCGGAGTCACTTTTCCTTGTGTTGATGTTTTTG
TTCTACTTGCAAATCATAAATTGTACAGCAAAAGTGCGCTTGTACAGATATCAGGACGAGTTGGACGAGCAGCTGAAAGA
CCAACAGGAGAATTGCTATTTTTACATGATGGAGCGACAAAAGCAATGAAAAAAGCTATTTCAGAAATAAAAGAGATGAA
TAAAAAAGGAGGATTCTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-397)

DEAD/DEAH box helicase (DEAD)

(97-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

61.827

98.843

0.611
  comFA/cflA Streptococcus pneumoniae D39

61.827

98.843

0.611
  comFA/cflA Streptococcus pneumoniae R6

61.827

98.843

0.611
  comFA/cflA Streptococcus pneumoniae TIGR4

61.827

98.843

0.611
  comFA/cflA Streptococcus mitis NCTC 12261

61.593

98.843

0.609
  comFA/cflA Streptococcus mitis SK321

61.124

98.843

0.604
  comFA Lactococcus lactis subsp. cremoris KW2

48.73

100

0.488
  comFA Latilactobacillus sakei subsp. sakei 23K

38.073

100

0.384

Multiple sequence alignment