Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   GPY01_RS14820 Genome accession   NZ_CP046839
Coordinates   2239704..2240288 (+) Length   194 a.a.
NCBI ID   WP_000902746.1    Uniprot ID   -
Organism   Vibrio cholerae strain 2011EL-1271     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2234704..2245288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY01_RS14805 oxyR 2235109..2236002 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  GPY01_RS14810 - 2236078..2238555 (-) 2478 WP_000668734.1 penicillin-binding protein 1A -
  GPY01_RS14815 pilM 2238698..2239714 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  GPY01_RS14820 pilN 2239704..2240288 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  GPY01_RS14825 pilO 2240281..2240871 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY01_RS14830 pilP 2240861..2241379 (+) 519 WP_000792033.1 pilus assembly protein PilP Machinery gene
  GPY01_RS14835 pilQ 2241405..2243120 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY01_RS14840 aroK 2243307..2243831 (+) 525 WP_000818616.1 shikimate kinase AroK -
  GPY01_RS14845 aroB 2243859..2244944 (+) 1086 WP_000439805.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22022.45 Da        Isoelectric Point: 9.7294

>NTDB_id=408693 GPY01_RS14820 WP_000902746.1 2239704..2240288(+) (pilN) [Vibrio cholerae strain 2011EL-1271]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQEGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=408693 GPY01_RS14820 WP_000902746.1 2239704..2240288(+) (pilN) [Vibrio cholerae strain 2011EL-1271]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCCGCCGGTGAATACTTGGGCGGACAAATGGCTTTGCAGCAAGAGC
GGATTGGTTATTTGCAACAACATATTTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAACGCAATAAAACCACTGAGTTTATGAACCAAATGCC
CAACCTGATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGAGGGAGGTGCACACAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

99.485

100

0.995
  pilN Vibrio campbellii strain DS40M4

60.452

91.237

0.552

Multiple sequence alignment