Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   GPY47_RS17065 Genome accession   NZ_CP046806
Coordinates   1899907..1900482 (-) Length   191 a.a.
NCBI ID   WP_017821732.1    Uniprot ID   A0A2I3CBW9
Organism   Vibrio alginolyticus strain 2439-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1894907..1905482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY47_RS17040 aroB 1895206..1896306 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  GPY47_RS17045 aroK 1896339..1896857 (-) 519 WP_005381319.1 shikimate kinase AroK -
  GPY47_RS17050 pilQ 1897045..1898790 (-) 1746 WP_005382765.1 type IV pilus secretin PilQ Machinery gene
  GPY47_RS17055 pilP 1898816..1899331 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  GPY47_RS17060 pilO 1899321..1899914 (-) 594 WP_017633532.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY47_RS17065 pilN 1899907..1900482 (-) 576 WP_017821732.1 PilN domain-containing protein Machinery gene
  GPY47_RS17070 pilM 1900466..1901482 (-) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene
  GPY47_RS17075 - 1901627..1904188 (+) 2562 WP_025768081.1 penicillin-binding protein 1A -
  GPY47_RS17080 oxyR 1904242..1905153 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22349.86 Da        Isoelectric Point: 7.4658

>NTDB_id=408107 GPY47_RS17065 WP_017821732.1 1899907..1900482(-) (pilN) [Vibrio alginolyticus strain 2439-01]
MLYRINLLPWRESQREEHRRRFISLVALGMLVAVGIQWGIGKFFEYQQDQQQERLDYLTHYIAELDQRIEAMKIAEQEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPKLATMLDNMERSAKLNDVEMHSIV
HGKARFGKEFQTFKVSFMFKVTEKKLEAQRG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=408107 GPY47_RS17065 WP_017821732.1 1899907..1900482(-) (pilN) [Vibrio alginolyticus strain 2439-01]
GTGCTTTATCGCATTAATTTACTTCCTTGGCGAGAGAGTCAAAGAGAAGAGCACCGACGTCGTTTCATCAGTTTGGTCGC
ATTAGGCATGTTGGTCGCTGTTGGCATCCAGTGGGGTATTGGAAAGTTTTTTGAGTACCAACAAGATCAACAACAGGAGC
GGCTCGATTACCTCACTCACTACATTGCCGAACTCGACCAGCGTATTGAAGCAATGAAAATTGCAGAGCAAGAGCACAGC
AAAATCCTCGAACGTCTAAAGGTCGTTGAAGGTTTACAAAACGGACGAAACAAAACGACCGAGTTCATGAACTTAATGCC
TGCCGTTATCCCTGAAGGTGTATATGTCGACAAGATCAAAATGAATGATCTGGAAATTGAGATCTCTGGGATCTCTGATA
GCACTCCAAAACTTGCCACGATGCTGGACAACATGGAGCGCTCGGCCAAGTTGAACGACGTAGAAATGCATTCCATCGTT
CACGGCAAAGCCCGTTTTGGGAAAGAGTTTCAAACATTCAAAGTGTCTTTCATGTTCAAAGTAACGGAGAAGAAATTGGA
GGCTCAACGTGGTTGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3CBW9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

82.199

100

0.822
  pilN Vibrio cholerae strain A1552

63.277

92.67

0.586

Multiple sequence alignment