Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY43_RS10525 Genome accession   NZ_CP046797
Coordinates   2270877..2271881 (-) Length   334 a.a.
NCBI ID   WP_158107812.1    Uniprot ID   -
Organism   Vibrio furnissii strain 2013V-1001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2265877..2276881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY43_RS10500 aroK 2266757..2267275 (-) 519 WP_004728569.1 shikimate kinase AroK -
  GPY43_RS10505 pilQ 2267463..2269187 (-) 1725 WP_041942471.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY43_RS10510 pilP 2269212..2269724 (-) 513 WP_154180461.1 pilus assembly protein PilP Machinery gene
  GPY43_RS10515 pilO 2269714..2270304 (-) 591 WP_055465836.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY43_RS10520 pilN 2270297..2270887 (-) 591 WP_055465837.1 PilN domain-containing protein Machinery gene
  GPY43_RS10525 pilM 2270877..2271881 (-) 1005 WP_158107812.1 type IV pilus assembly protein PilM Machinery gene
  GPY43_RS10530 - 2272026..2274506 (+) 2481 WP_158107813.1 PBP1A family penicillin-binding protein -
  GPY43_RS10535 oxyR 2274593..2275489 (-) 897 WP_014204332.1 DNA-binding transcriptional regulator OxyR -
  GPY43_RS10540 - 2275633..2276367 (+) 735 WP_004728561.1 glutathione peroxidase -
  GPY43_RS10545 - 2276491..2276754 (+) 264 WP_158107814.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36892.17 Da        Isoelectric Point: 6.5009

>NTDB_id=407957 GPY43_RS10525 WP_158107812.1 2270877..2271881(-) (pilM) [Vibrio furnissii strain 2013V-1001]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSDDIFADNHTLDYQKIVKKLKELKKGLPLFSRKVALAVPDNA
VISKVLQIDSDLDASEQEFAVYQAFSHQSPFPIDDLSLDFVPSGDNPAGRSASTLYQVYATRREVVDNRLSVASKAGFEP
LLLDVQVHGLLQIWQWVSRAQQRDDWMLVDVGLTQTTLCMDFADKAPFYKDIPLGTRQWHADPTPHPVPLSEPPMAEVNE
LVERIARQVQLLLSLQGHQALGGIWLSGGGATTEGLSAALAARLNLPCELFHPLTGFVTRRRMRARVPDDLQRFSTAAGL
ALRGLNWLERDHGA

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=407957 GPY43_RS10525 WP_158107812.1 2270877..2271881(-) (pilM) [Vibrio furnissii strain 2013V-1001]
ATGGGTAAATCATTAGTTACAGGTGTCGATATCGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAGGGCGAATC
GTATTCGCTGGTCGGCTACCAAGAAATAGTCGTATCCGACGATATTTTCGCTGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGATTACCGCTATTTAGTCGCAAAGTTGCGCTGGCTGTGCCGGATAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGCGATTTAGATGCCAGCGAACAAGAATTTGCTGTCTATCAGGCGTTTTCCCA
TCAATCGCCGTTTCCCATCGATGATCTGAGTTTGGATTTCGTACCAAGCGGCGATAACCCCGCTGGGCGCAGTGCCAGCA
CCCTGTATCAGGTGTACGCCACGCGACGCGAAGTCGTCGATAACCGGTTATCGGTGGCGAGCAAAGCGGGCTTTGAGCCG
CTGCTGCTCGATGTGCAGGTGCATGGGTTGCTCCAGATTTGGCAATGGGTGAGTCGCGCGCAGCAGCGCGACGATTGGAT
GCTGGTGGACGTCGGGTTGACGCAAACCACGTTATGCATGGATTTCGCCGATAAAGCGCCCTTCTATAAAGACATTCCGC
TGGGAACGCGTCAATGGCATGCCGACCCCACGCCCCATCCTGTGCCGCTGAGCGAGCCACCGATGGCTGAAGTCAACGAG
CTGGTTGAGCGAATTGCGCGCCAAGTGCAACTGCTGTTGTCCTTGCAGGGCCATCAAGCGCTGGGCGGGATTTGGCTGTC
GGGCGGTGGGGCGACGACGGAAGGCCTCAGCGCGGCTTTGGCTGCGCGACTGAATCTGCCGTGTGAACTGTTTCATCCGC
TGACGGGATTTGTGACGCGCCGCCGCATGCGAGCGCGTGTGCCAGACGATCTGCAACGCTTTAGTACGGCGGCCGGCTTG
GCGCTGCGCGGACTCAACTGGTTGGAGCGTGACCATGGTGCATGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-218)

Type IV pilus assembly protein PilM; (PilM_2)

(239-324)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

64.307

100

0.653
  pilM Vibrio campbellii strain DS40M4

45.266

100

0.458

Multiple sequence alignment