Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   H0E72_RS09260 Genome accession   NZ_CP059003
Coordinates   1929557..1930567 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain 2D     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1924557..1935567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0E72_RS09230 (H0E72_09230) nudB 1926281..1926733 (+) 453 WP_077526652.1 dihydroneopterin triphosphate diphosphatase -
  H0E72_RS09235 (H0E72_09235) yebC 1926762..1927502 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  H0E72_RS09240 (H0E72_09240) ruvC 1927537..1928058 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  H0E72_RS09245 (H0E72_09245) yebB 1928060..1928662 (-) 603 WP_001024944.1 YebB family permuted papain-like enzyme -
  H0E72_RS09250 (H0E72_09250) yobI 1928733..1928798 (+) 66 WP_001386853.1 stress response small protein YobI -
  H0E72_RS09255 (H0E72_09255) ruvA 1928937..1929548 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  H0E72_RS09260 (H0E72_09260) ruvB 1929557..1930567 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  H0E72_RS09265 (H0E72_09265) znuB 1930714..1931499 (-) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  H0E72_RS09270 (H0E72_09270) znuC 1931496..1932251 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  H0E72_RS09275 (H0E72_09275) znuA 1932330..1933262 (+) 933 WP_024175798.1 zinc ABC transporter substrate-binding protein ZnuA -
  H0E72_RS09280 (H0E72_09280) mepM 1933278..1934600 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=407957 H0E72_RS09260 WP_000568522.1 1929557..1930567(+) (ruvB) [Escherichia coli strain 2D]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=407957 H0E72_RS09260 WP_000568522.1 1929557..1930567(+) (ruvB) [Escherichia coli strain 2D]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAACCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGTGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCTAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGGGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATTATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCTAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCTGCCATTGGTGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCACCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment