Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MS10711_RS16140 Genome accession   NZ_CP058922
Coordinates   3287349..3288158 (-) Length   269 a.a.
NCBI ID   WP_001305093.1    Uniprot ID   -
Organism   Escherichia coli strain MS10711     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3282349..3293158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS10711_RS16125 (MS10711_3145) gspD 3283806..3285866 (-) 2061 WP_000498847.1 type II secretion system secretin GspD -
  MS10711_RS16130 (MS10711_3146) gspC 3285896..3286855 (-) 960 WP_001305091.1 type II secretion system protein GspC -
  MS10711_RS16135 (MS10711_3147) gspS2 3286873..3287283 (-) 411 WP_001305092.1 type II secretion system pilot lipoprotein GspS-beta -
  MS10711_RS16140 (MS10711_3148) pilD 3287349..3288158 (-) 810 WP_001305093.1 prepilin peptidase PppA Machinery gene
  MS10711_RS16145 (MS10711_3149) sslE 3288288..3292844 (-) 4557 WP_001034480.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29448.04 Da        Isoelectric Point: 8.3801

>NTDB_id=407730 MS10711_RS16140 WP_001305093.1 3287349..3288158(-) (pilD) [Escherichia coli strain MS10711]
MLFDVFQQYSAGMPILATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSTQSKINLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=407730 MS10711_RS16140 WP_001305093.1 3287349..3288158(-) (pilD) [Escherichia coli strain MS10711]
ATGCTTTTTGATGTTTTTCAGCAATACTCCGCGGGGATGCCCATACTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGCCGAGTACGC
AGTCAAAAATAAACCTGGCGCTGCCACGCTCGCACTGTCCGCATTGCCAGCAGACCATCCGCGTTCGTGACAATATTCCG
CTGCTCTCATGGTTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTGTGGCCAGAAAGTGGATGGGGGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCGTCATTGACCTCGATAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACC
GGGCTGATTGCGGCATGGGCGCAACAGAGTCCGTTAACGCTACAAGACGCAGTTACCGGCGTCCTGGTGGGGTTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTTTTCGCTG
CGTTAGGTGGCTGGGTGGGGCCGTTGTCGCTACCCAATGTTGCTTTAATCGCATCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.318

99.257

0.45
  pilD Vibrio campbellii strain DS40M4

41.328

100

0.416
  pilD Acinetobacter nosocomialis M2

38.113

98.513

0.375
  pilD Neisseria gonorrhoeae MS11

39.841

93.309

0.372
  pilD Acinetobacter baumannii D1279779

37.736

98.513

0.372

Multiple sequence alignment