Detailed information NTG0407732

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: MS10711_RS16455
• Genome accession: NZ_CP058922
• Coordinates: 3358166..3358825 (+)
• Length: 219 a.a.
• NCBI ID: WP_001221493.1
• Uniprot ID: Q3YXL4
• Organism: Escherichia coli strain MS10711
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3353166..3363825

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MS10711_RS16430 (MS10711_3207)	ygiS	3354449..3356056 (-)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
MS10711_RS16435 (MS10711_3208)	mqsA	3356189..3356584 (-)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
MS10711_RS16440 (MS10711_3209)	mqsR	3356586..3356882 (-)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
MS10711_RS16445 (MS10711_3210)	ygiV	3357087..3357569 (-)	483	WP_000183505.1	GyrI-like domain-containing protein	-
MS10711_RS16450 (MS10711_3211)	ygiW	3357622..3358014 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
MS10711_RS16455 (MS10711_3212)	ciaR	3358166..3358825 (+)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
MS10711_RS16460 (MS10711_3213)	qseC	3358822..3360171 (+)	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
MS10711_RS16465	ygiZ	3360217..3360549 (-)	333	WP_000917685.1	DUF2645 family protein	-
MS10711_RS16470 (MS10711_3214)	mdaB	3360868..3361449 (+)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
MS10711_RS16475 (MS10711_3215)	ygiN	3361480..3361794 (+)	315	WP_000958598.1	putative quinol monooxygenase	-
MS10711_RS16480 (MS10711_3216)	parE	3361842..3363734 (-)	1893	WP_001774047.1	DNA topoisomerase IV subunit B	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24687.63 Da
• Isoelectric Point: 6.9850

>NTDB_id=407732 MS10711_RS16455 WP_001221493.1 3358166..3358825(+) (ciaR) [Escherichia coli strain MS10711]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=407732 MS10711_RS16455 WP_001221493.1 3358166..3358825(+) (ciaR) [Escherichia coli strain MS10711]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: Q3YXL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365