Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPX90_RS10975 Genome accession   NZ_CP046752
Coordinates   1252972..1254699 (-) Length   575 a.a.
NCBI ID   WP_407046411.1    Uniprot ID   -
Organism   Photobacterium damselae strain 9046-81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1247972..1259699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX90_RS10950 - 1248358..1248555 (+) 198 WP_036764707.1 DUF2970 domain-containing protein -
  GPX90_RS10955 dam 1248539..1249381 (-) 843 WP_065172417.1 adenine-specific DNA-methyltransferase -
  GPX90_RS10960 - 1249489..1251000 (-) 1512 WP_158135420.1 SPOR domain-containing protein -
  GPX90_RS10965 aroB 1251015..1252094 (-) 1080 WP_005302661.1 3-dehydroquinate synthase -
  GPX90_RS10970 aroK 1252124..1252642 (-) 519 WP_036765246.1 shikimate kinase AroK -
  GPX90_RS10975 pilQ 1252972..1254699 (-) 1728 WP_407046411.1 type IV pilus secretin PilQ Machinery gene
  GPX90_RS10980 - 1254723..1255238 (-) 516 WP_036764704.1 pilus assembly protein PilP -
  GPX90_RS10985 - 1255247..1255837 (-) 591 WP_158135424.1 type 4a pilus biogenesis protein PilO -
  GPX90_RS10990 - 1255834..1256475 (-) 642 WP_068945318.1 PilN domain-containing protein -
  GPX90_RS20150 pilM 1256472..1257080 (-) 609 Protein_1070 type IV pilus biogenesis protein PilM -
  GPX90_RS20155 pilM 1257113..1257469 (-) 357 WP_265094733.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62701.18 Da        Isoelectric Point: 4.8489

>NTDB_id=407150 GPX90_RS10975 WP_407046411.1 1252972..1254699(-) (pilQ) [Photobacterium damselae strain 9046-81]
MKRMLSVSLGWQCCWVVMLCCWLGLSQAAIAAGMNIVNNQITDLDFKRDNRGRGVLTVQLAKPQSLVDMRRSGKQLHIDI
FDTAIKDDMLYVLDVVDFATVVRSIETFRTEKGSKLILNMAGVFDYDYQLKGSRLEVVLNAQKKKASSTAKAKQGKIDYK
GKPISINFQDVPVRNVLQLVAEYNDFNLVVSDTVQGNVTLRLDDVPWPQVLDIILQSKGLDKRVQGSVVLIAPKSELATN
ERQEMEAKRKEDELASLRSELIQINYAKAGDISELLLGTKDGVSMLSQRGSLHVDERTNAIIIKDIPESIDSIEEIITAL
DIPVKQVQIEARIVTVDEGDIDELGVRWGVVNQNGSTTIGGSIEGNLASSGLLGDDATVDDFLNVNLGAITPGASSIAFQ
VAKLGDILLDLELSALQAESKAEIISSPRLVTTNKKAAYIEQGTEIPYLEAASSGAVSVDFKKAVLSLMVTPQITPDDKL
VLDLLVTQDKVGETVVTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIYQHQVNNSVRKVPLLGDIPGLGVLFRHKLQEMG
KRELLIFVTPKIITQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=407150 GPX90_RS10975 WP_407046411.1 1252972..1254699(-) (pilQ) [Photobacterium damselae strain 9046-81]
ATGAAAAGGATGTTATCGGTCAGCTTGGGTTGGCAATGTTGTTGGGTAGTTATGTTGTGCTGTTGGCTTGGTCTCTCCCA
AGCTGCCATTGCTGCTGGCATGAATATTGTGAATAACCAAATCACCGACTTAGATTTTAAGCGTGATAACCGTGGACGAG
GCGTGCTCACTGTTCAGTTAGCCAAACCTCAATCTTTGGTTGATATGCGCCGTTCAGGGAAGCAATTACACATCGATATC
TTTGATACCGCGATTAAAGATGACATGTTGTATGTACTCGATGTGGTCGACTTTGCAACGGTTGTTCGTTCGATTGAAAC
ATTTCGCACTGAGAAAGGCAGCAAACTTATTCTCAATATGGCTGGCGTGTTTGATTATGATTACCAATTAAAAGGCTCTC
GTCTTGAAGTTGTTTTGAACGCTCAGAAAAAGAAAGCCAGCAGTACAGCTAAGGCTAAGCAAGGAAAAATTGATTATAAA
GGTAAGCCCATCTCCATTAACTTCCAAGATGTTCCTGTGCGTAACGTGTTGCAGTTGGTTGCCGAATATAATGATTTTAA
TCTCGTGGTTTCCGATACTGTGCAGGGTAATGTGACCTTACGTCTTGATGATGTGCCTTGGCCACAAGTGCTTGATATTA
TTTTGCAATCAAAAGGCTTGGATAAACGAGTTCAAGGTTCTGTGGTACTGATTGCGCCAAAATCTGAGTTGGCAACCAAT
GAGCGTCAAGAGATGGAAGCCAAACGTAAAGAAGATGAGTTAGCTTCGCTTCGTTCGGAGCTGATTCAAATTAATTACGC
TAAAGCAGGAGATATTTCAGAGCTTTTACTCGGAACGAAAGATGGAGTCAGTATGCTATCTCAACGCGGGTCTTTGCATG
TTGATGAGCGTACTAATGCCATTATCATCAAAGATATCCCTGAGAGTATTGATAGCATTGAAGAGATTATCACGGCACTA
GATATTCCCGTTAAACAGGTTCAGATAGAAGCTCGTATTGTTACTGTTGATGAAGGGGATATTGACGAGTTAGGGGTACG
TTGGGGTGTCGTTAATCAAAACGGTAGTACAACCATTGGGGGCTCAATTGAAGGTAACTTGGCCAGTTCGGGCTTATTGG
GTGATGACGCGACTGTTGATGATTTCTTAAATGTAAATTTAGGAGCCATTACTCCAGGGGCATCGAGTATTGCATTTCAG
GTGGCAAAACTGGGCGATATTTTGCTCGATCTTGAATTATCCGCTCTACAGGCAGAATCGAAAGCAGAGATTATTTCTAG
TCCTCGATTAGTAACCACCAATAAAAAAGCAGCTTACATCGAACAAGGTACAGAGATCCCTTATTTAGAAGCGGCATCTA
GTGGTGCGGTATCGGTAGACTTTAAAAAGGCCGTATTGAGTTTGATGGTCACACCACAAATTACCCCTGATGATAAATTG
GTGCTCGATCTGCTGGTAACGCAAGATAAAGTAGGGGAAACAGTTGTTACCGGAACTGGAGAAGCGGTCGCTATCGATAC
TCAACGAATTGGCACGCAAGTTCTGGTCAATAATGGCGAGACTGTAGTCTTAGGGGGTATCTATCAGCATCAGGTGAATA
ATTCTGTCCGTAAAGTCCCTTTGTTGGGCGATATTCCAGGATTGGGCGTATTGTTCCGTCATAAGCTGCAAGAGATGGGC
AAGCGTGAACTGTTGATTTTCGTCACACCCAAAATTATCACCCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-326)

Bacterial type II and III secretion system protein (Secretin)

(415-573)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

61.121

99.304

0.607
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

61.121

99.304

0.607
  pilQ Vibrio campbellii strain DS40M4

61.111

97.043

0.593
  pilQ Legionella pneumophila strain Lp02

42.647

94.609

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.647

94.609

0.403
  pilQ Pseudomonas aeruginosa PAK

40.925

97.739

0.4
  comQ Acinetobacter baylyi ADP1

40.212

98.609

0.397
  pilQ Acinetobacter baumannii D1279779

39.138

100

0.395
  pilQ Acinetobacter baumannii strain A118

39.138

100

0.395

Multiple sequence alignment