Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPX93_RS11890 Genome accession   NZ_CP046749
Coordinates   2605968..2606984 (-) Length   338 a.a.
NCBI ID   WP_123163007.1    Uniprot ID   -
Organism   Vibrio cholerae strain 2015V-1118     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2600968..2611984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX93_RS11865 aroK 2601851..2602375 (-) 525 WP_000818616.1 shikimate kinase AroK -
  GPX93_RS11870 pilQ 2602562..2604277 (-) 1716 WP_162808987.1 type IV pilus secretin PilQ family protein Machinery gene
  GPX93_RS11875 pilP 2604303..2604821 (-) 519 WP_123163006.1 pilus assembly protein PilP Machinery gene
  GPX93_RS11880 pilO 2604811..2605401 (-) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX93_RS11885 pilN 2605394..2605978 (-) 585 WP_162811704.1 PilN domain-containing protein Machinery gene
  GPX93_RS11890 pilM 2605968..2606984 (-) 1017 WP_123163007.1 type IV pilus assembly protein PilM Machinery gene
  GPX93_RS11895 - 2607127..2609604 (+) 2478 WP_184482924.1 penicillin-binding protein 1A -
  GPX93_RS11900 oxyR 2609680..2610573 (-) 894 WP_001914100.1 DNA-binding transcriptional regulator OxyR -
  GPX93_RS11905 - 2610713..2611444 (+) 732 WP_184482927.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37682.40 Da        Isoelectric Point: 6.9294

>NTDB_id=407116 GPX93_RS11890 WP_123163007.1 2605968..2606984(-) (pilM) [Vibrio cholerae strain 2015V-1118]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVQVTEKSLVRSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQCFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=407116 GPX93_RS11890 WP_123163007.1 2605968..2606984(-) (pilM) [Vibrio cholerae strain 2015V-1118]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCGCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCCAAGTGACGGAAAAAAGCCTCGTCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGTGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.112

100

0.991
  pilM Vibrio campbellii strain DS40M4

48.225

100

0.482

Multiple sequence alignment