Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY00_RS07635 Genome accession   NZ_CP046747
Coordinates   1636290..1637306 (+) Length   338 a.a.
NCBI ID   WP_158128539.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3541-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1631290..1642306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY00_RS07620 - 1631830..1632561 (-) 732 WP_000495226.1 glutathione peroxidase -
  GPY00_RS07625 oxyR 1632701..1633594 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  GPY00_RS07630 - 1633670..1636147 (-) 2478 WP_199252369.1 penicillin-binding protein 1A -
  GPY00_RS07635 pilM 1636290..1637306 (+) 1017 WP_158128539.1 type IV pilus assembly protein PilM Machinery gene
  GPY00_RS07640 pilN 1637296..1637880 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  GPY00_RS07645 pilO 1637873..1638463 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY00_RS07650 pilP 1638453..1638971 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  GPY00_RS07655 pilQ 1638997..1640712 (+) 1716 WP_187386856.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY00_RS07660 aroK 1640899..1641423 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37776.55 Da        Isoelectric Point: 7.6229

>NTDB_id=407059 GPY00_RS07635 WP_158128539.1 1636290..1637306(+) (pilM) [Vibrio cholerae strain 3541-04]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDRDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=407059 GPY00_RS07635 WP_158128539.1 1636290..1637306(+) (pilM) [Vibrio cholerae strain 3541-04]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGAGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCATTGCCTTGTGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.704

100

0.997
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485

Multiple sequence alignment