Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPW86_RS11440 Genome accession   NZ_CP046742
Coordinates   1219365..1220381 (-) Length   338 a.a.
NCBI ID   WP_123011176.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3523-03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1214365..1225381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW86_RS11415 aroK 1215248..1215772 (-) 525 WP_000818616.1 shikimate kinase AroK -
  GPW86_RS11420 pilQ 1215959..1217674 (-) 1716 WP_000788428.1 type IV pilus secretin PilQ Machinery gene
  GPW86_RS11425 pilP 1217700..1218218 (-) 519 WP_123011174.1 pilus assembly protein PilP Machinery gene
  GPW86_RS11430 pilO 1218208..1218798 (-) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPW86_RS11435 pilN 1218791..1219375 (-) 585 WP_184476137.1 PilN domain-containing protein Machinery gene
  GPW86_RS11440 pilM 1219365..1220381 (-) 1017 WP_123011176.1 type IV pilus assembly protein PilM Machinery gene
  GPW86_RS11445 - 1220524..1223001 (+) 2478 WP_057555154.1 penicillin-binding protein 1A -
  GPW86_RS11450 oxyR 1223076..1223969 (-) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  GPW86_RS11455 - 1224109..1224840 (+) 732 WP_000495226.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37735.49 Da        Isoelectric Point: 7.3328

>NTDB_id=406963 GPW86_RS11440 WP_123011176.1 1219365..1220381(-) (pilM) [Vibrio cholerae strain 3523-03]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKVGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=406963 GPW86_RS11440 WP_123011176.1 1219365..1220381(-) (pilM) [Vibrio cholerae strain 3523-03]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTACTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGTTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGTGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTCTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCATTGCCTTGTGAAG
TACTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCAGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.704

100

0.997
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485

Multiple sequence alignment