Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPW86_RS11420 Genome accession   NZ_CP046742
Coordinates   1215959..1217674 (-) Length   571 a.a.
NCBI ID   WP_000788428.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3523-03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1210959..1222674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW86_RS11400 - 1211702..1212535 (-) 834 WP_000744680.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  GPW86_RS11405 - 1212605..1214080 (-) 1476 WP_123011173.1 AAA family ATPase -
  GPW86_RS11410 aroB 1214135..1215220 (-) 1086 WP_069504185.1 3-dehydroquinate synthase -
  GPW86_RS11415 aroK 1215248..1215772 (-) 525 WP_000818616.1 shikimate kinase AroK -
  GPW86_RS11420 pilQ 1215959..1217674 (-) 1716 WP_000788428.1 type IV pilus secretin PilQ Machinery gene
  GPW86_RS11425 pilP 1217700..1218218 (-) 519 WP_123011174.1 pilus assembly protein PilP Machinery gene
  GPW86_RS11430 pilO 1218208..1218798 (-) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPW86_RS11435 pilN 1218791..1219375 (-) 585 WP_184476137.1 PilN domain-containing protein Machinery gene
  GPW86_RS11440 pilM 1219365..1220381 (-) 1017 WP_123011176.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62354.47 Da        Isoelectric Point: 4.5785

>NTDB_id=406959 GPW86_RS11420 WP_000788428.1 1215959..1217674(-) (pilQ) [Vibrio cholerae strain 3523-03]
MKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGTIESNLWQQGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=406959 GPW86_RS11420 WP_000788428.1 1215959..1217674(-) (pilQ) [Vibrio cholerae strain 3523-03]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTACTTGATGTGAAAGATTTTTCGACCCCTGTTGAGAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTTGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATACCTTGAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCCGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTTATCTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAGCGGGTCGATGGCAATGTGATTTTGATTGCGCCAAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTCAACATGTTGTCTGAACGAGGTTCGATCAGCATCGATGAACGAACTAACT
CGCTATTGATCCGAGAGCTGCCCGATAACATTGCCGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCTGGGGGTACGCTGGGGCGTAATGTCGAC
CAATGGTAGTCACTCCGTGGGTGGAACCATTGAAAGTAACTTATGGCAGCAAGGTTTATTGGCAGATGATGAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAATTGGGC
TCAGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAATCATCTCTAGCCCTCGTTTGAT
TACCACTAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTAAGCTTGAAGGTGACACCACAAATCACCCCAGATAACCGCTTGGTGCTCGACTTG
AGTGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGTACGGGTGAAGCGGTATCGATCGATACTCAGCGTATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGGGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

99.65

100

0.997
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

99.65

100

0.997
  pilQ Vibrio campbellii strain DS40M4

78.364

96.322

0.755
  pilQ Legionella pneumophila strain ERS1305867

42.963

94.571

0.406
  pilQ Legionella pneumophila strain Lp02

42.963

94.571

0.406
  comQ Acinetobacter baylyi ADP1

39.161

100

0.392
  pilQ Acinetobacter baumannii D1279779

37.819

100

0.389
  pilQ Acinetobacter baumannii strain A118

37.819

100

0.389
  pilQ Pseudomonas aeruginosa PAK

39.312

96.673

0.38

Multiple sequence alignment