Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPW70_RS06350 Genome accession   NZ_CP046740
Coordinates   304039..305055 (+) Length   338 a.a.
NCBI ID   WP_000520444.1    Uniprot ID   A0A0F0B833
Organism   Vibrio cholerae strain 3566-06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 299039..310055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW70_RS06335 - 299579..300310 (-) 732 WP_000495223.1 glutathione peroxidase -
  GPW70_RS06340 oxyR 300450..301343 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  GPW70_RS06345 - 301419..303896 (-) 2478 WP_032472750.1 penicillin-binding protein 1A -
  GPW70_RS06350 pilM 304039..305055 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  GPW70_RS06355 pilN 305045..305629 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  GPW70_RS06360 pilO 305622..306212 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPW70_RS06365 pilP 306202..306720 (+) 519 WP_032472751.1 pilus assembly protein PilP Machinery gene
  GPW70_RS06370 pilQ 306746..308461 (+) 1716 WP_032472752.1 type IV pilus secretin PilQ Machinery gene
  GPW70_RS06375 aroK 308648..309172 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37707.44 Da        Isoelectric Point: 7.3328

>NTDB_id=406904 GPW70_RS06350 WP_000520444.1 304039..305055(+) (pilM) [Vibrio cholerae strain 3566-06]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=406904 GPW70_RS06350 WP_000520444.1 304039..305055(+) (pilM) [Vibrio cholerae strain 3566-06]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCGCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTAGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTCTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F0B833

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

100

100

1
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485

Multiple sequence alignment