Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPW74_RS08390 Genome accession   NZ_CP046737
Coordinates   1870761..1871777 (-) Length   338 a.a.
NCBI ID   WP_123012524.1    Uniprot ID   -
Organism   Vibrio cholerae strain 2015V-1126     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1865761..1876777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW74_RS08365 aroK 1866644..1867168 (-) 525 WP_000818616.1 shikimate kinase AroK -
  GPW74_RS08370 pilQ 1867355..1869070 (-) 1716 WP_162806863.1 type IV pilus secretin PilQ family protein Machinery gene
  GPW74_RS08375 pilP 1869096..1869614 (-) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  GPW74_RS08380 pilO 1869604..1870194 (-) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPW74_RS08385 pilN 1870187..1870771 (-) 585 WP_172776179.1 PilN domain-containing protein Machinery gene
  GPW74_RS08390 pilM 1870761..1871777 (-) 1017 WP_123012524.1 type IV pilus assembly protein PilM Machinery gene
  GPW74_RS08395 - 1871920..1874397 (+) 2478 WP_162813371.1 penicillin-binding protein 1A -
  GPW74_RS08400 oxyR 1874474..1875367 (-) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  GPW74_RS08405 - 1875507..1876238 (+) 732 WP_000495223.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37625.33 Da        Isoelectric Point: 7.6228

>NTDB_id=406867 GPW74_RS08390 WP_123012524.1 1870761..1871777(-) (pilM) [Vibrio cholerae strain 2015V-1126]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTGKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNSFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=406867 GPW74_RS08390 WP_123012524.1 1870761..1871777(-) (pilM) [Vibrio cholerae strain 2015V-1126]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGGAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCATTGCCTTGCGAAG
TGCTCAATTCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.408

100

0.994
  pilM Vibrio campbellii strain DS40M4

48.225

100

0.482

Multiple sequence alignment