Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   HYI45_RS00220 Genome accession   NZ_CP058714
Coordinates   22061..23485 (+) Length   474 a.a.
NCBI ID   WP_039052523.1    Uniprot ID   -
Organism   Escherichia coli strain M172-1     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 17061..28485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HYI45_RS00170 - 17211..17507 (+) 297 WP_049038791.1 phage anti-RecBCD protein -
  HYI45_RS00175 - 17518..17682 (+) 165 WP_001214456.1 DUF2737 family protein -
  HYI45_RS00180 - 17679..18128 (+) 450 WP_049038793.1 hypothetical protein -
  HYI45_RS00185 - 18125..18577 (+) 453 WP_049038794.1 ead/Ea22-like family protein -
  HYI45_RS26900 - 18531..18842 (+) 312 Protein_37 hypothetical protein -
  HYI45_RS26905 - 18831..19166 (+) 336 Protein_38 hypothetical protein -
  HYI45_RS00195 - 19163..19726 (+) 564 WP_239795001.1 DUF551 domain-containing protein -
  HYI45_RS00200 - 19823..20002 (+) 180 WP_001277767.1 Eag protein -
  HYI45_RS00205 - 20105..20449 (+) 345 WP_239795002.1 hypothetical protein -
  HYI45_RS00210 - 20529..20720 (+) 192 WP_000132739.1 AlpA family phage regulatory protein -
  HYI45_RS00215 - 20701..21879 (-) 1179 WP_239795003.1 site-specific integrase -
  HYI45_RS00220 sbcB 22061..23485 (+) 1425 WP_039052523.1 exodeoxyribonuclease I Machinery gene
  HYI45_RS00225 tsuB 23528..23755 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  HYI45_RS00230 tsuA 23769..24827 (-) 1059 WP_000492349.1 thiosulfate utilization transporter TsuA/YeeE -
  HYI45_RS00235 plaP 25006..26364 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  HYI45_RS00240 yoeI 26354..26416 (-) 63 WP_010723108.1 membrane protein YoeI -
  HYI45_RS00245 yeeY 26631..27560 (-) 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
  HYI45_RS00250 yeeZ 27606..28430 (-) 825 WP_000754737.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54289.53 Da        Isoelectric Point: 5.2440

>NTDB_id=406663 HYI45_RS00220 WP_039052523.1 22061..23485(+) (sbcB) [Escherichia coli strain M172-1]
MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDAE
RLGIARQHCLDNLKVLRENPQVRDKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDDNEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQLYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=406663 HYI45_RS00220 WP_039052523.1 22061..23485(+) (sbcB) [Escherichia coli strain M172-1]
ATGACAGACACAGATAAACAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCGG
ATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAA
GCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGATGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGTGGC
AATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGACAT
TTCACCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACGCCG
CCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGACGCTGAG
CGTTTAGGGATTGCTCGTCAACACTGCCTGGATAACCTGAAAGTGTTGCGCGAAAACCCGCAAGTACGCGACAAAGTTGT
GGCAATTTTTGCAGAAGCTGAACCGTTTACACCATCAGATAATGTAGATGCACAGCTTTATAATGGCTTTTTTAGCGACG
CCGATCGTGCAGCAATGAAAATCGTGCTGGAGACTGAACCGCGTAATTTACCGGCTCTGGATATCACCTTTGTTGATAAG
CGTATCGAGAAACTCCTGTTCAACTACCGCGCCCGCAACTTCCCCGGCACGTTAGATGACAACGAGCAGCAACGCTGGCT
TGAGCACCGCCGCCAGGTCTTCACACCCGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCTGTATG
CCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(12-187)

Exonuclease C-terminal (ExoI_SH3)

(211-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

59.149

99.156

0.586

Multiple sequence alignment