Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   GPA07_RS14525 Genome accession   NZ_CP046653
Coordinates   2779669..2783373 (-) Length   1234 a.a.
NCBI ID   WP_157292868.1    Uniprot ID   -
Organism   Bacillus sp. ms-22     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2774669..2788373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPA07_RS14510 (GPA07_14510) - 2774757..2775056 (-) 300 WP_008355148.1 hypothetical protein -
  GPA07_RS14515 (GPA07_14515) sbcC 2775075..2778470 (-) 3396 WP_008355145.1 exonuclease subunit SbcC -
  GPA07_RS14520 (GPA07_14520) sbcD 2778467..2779642 (-) 1176 WP_008355143.1 exonuclease subunit SbcD -
  GPA07_RS14525 (GPA07_14525) addA 2779669..2783373 (-) 3705 WP_157292868.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  GPA07_RS14530 (GPA07_14530) addB 2783351..2786860 (-) 3510 WP_008355139.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  GPA07_RS14535 (GPA07_14535) - 2787028..2787474 (+) 447 WP_008355136.1 ferritin-like domain-containing protein -
  GPA07_RS14540 (GPA07_14540) - 2787670..2788080 (-) 411 WP_008355134.1 DoxX family protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142807.99 Da        Isoelectric Point: 5.1329

>NTDB_id=406447 GPA07_RS14525 WP_157292868.1 2779669..2783373(-) (addA) [Bacillus sp. ms-22]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEQPIDVDRLLVVTFTNASAAEMKHRITEA
LEKELSKNPGSLHIRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLEQA
LTLSKEPGGPAPRTENFIDDLEQVNELITNQDDFEKLYELVPNINFKRLKTCKGDDYDPVLVEKATDARNQAKKQLEKLK
DEYFMRSPQQHLKSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAKQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGERPAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFKRDSKDTGKRIDLSKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPQSEDTTTEMLLVHLDQHEAESDEEREELETVQFEARVIARK
IKELVEQPFQVYDAKQKITRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVVLSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLEQFFFLLRKWRDFSMTHSVAELIWEVY
RDTHYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYTTLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAANGEEWLLPDFERYQAKTYLDFIGPALIRHQAMKPILEESRDVVLSHPSSFTITFHQASDL
LKEDVSLEKKKQDEVMQALMEGVPVEGYGDYEEEVKERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKPR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSAEAYDESRIQELLDSLKERDLLTDEQIDSINREGIAAFFSTSIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIEVEGEPVLIQGMIDCLFEADGKLYLLDYKTDRVSGRFSGGLEETEAMLKKRYE
TQIALYARAVERLTSRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=406447 GPA07_RS14525 WP_157292868.1 2779669..2783373(-) (addA) [Bacillus sp. ms-22]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGATCAATGGGAAGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCTGCGGCGGCAGGGTCAGGAAAAACGGCTGTCTTGGTTGAACGGCTGATTCGAAAAATGACCCGGCCAGAGCAGC
CAATTGATGTGGATCGCCTGCTCGTTGTGACTTTTACGAATGCATCTGCGGCAGAAATGAAGCACCGGATCACTGAAGCC
CTTGAAAAGGAATTATCCAAAAATCCTGGGTCACTTCATATAAGAAGACAGCTGTCCCTCATGAATCGTGCCAATATTTC
TACCTTGCACTCCTTTTGTTTACAAGTTCTCCGTACCTTTTATTATGAGATTGACCTTGATCCAGGCTTTCGTTTAGCCG
ATCAAACAGAAGGAGAATTATTAGGAGACGAAGTGTTAGATGAGCTGTTTGAGGATGAGTATAAGGCTGGAAAGCCAGCA
TTCTTTGAACTGGTTGACCGTTACACATCAGATCGTCATGATTTAGATCTTCAATGGCTTGTAAAAAGAATATATGAGTT
TTCTAGATCGCATCCATCGCCTGAACAATGGATGCGGGCATTCCTATCTCTCTATGATGTGGATGCACAAACAAAAGTAG
AAGAATTACCATTTTACCCTTATATAAAAGAGGATCTTTCTCTCGTTTTAAGAAGCTGTCAGGAACTGCTTGAACAAGCT
CTGACCCTATCAAAAGAGCCGGGCGGCCCAGCACCGAGAACAGAGAATTTTATAGATGATTTAGAGCAAGTGAATGAATT
AATCACAAATCAAGATGATTTTGAGAAACTTTATGAGCTCGTACCGAACATCAATTTTAAAAGGCTAAAGACGTGTAAAG
GGGACGACTATGACCCTGTTCTCGTAGAAAAAGCTACAGATGCACGCAATCAAGCCAAAAAACAATTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCTCAGCAGCATCTAAAAAGCTTGGCTGAAATGAAGCCAGTTGTAGAAACACTTGTGGA
ACTTGTCATCCAATTTGGAGAGCGTTTCGAAAGAGCAAAACAAGAGAAATCAATCGTAGACTTTTCGGATTTAGAGCATT
ATTGTTTACGCATCTTAGCAAAGCAGGATGCGGAAGGACATCTAATTGAAACAGAAGCAGCCAAATACTATCAACAGCAA
TTTGAAGAAGTGCTCGTTGATGAATACCAAGATACAAACCTTGTCCAAGAAACGATTTTAAAGCTTGTATCTAAAGGGGA
ACGTCCTGCAGAAGGGAATCTCTTTATGGTTGGTGATGTGAAGCAGTCTATTTATCGCTTTAGACTTGCTGAGCCGATGC
TCTTTTTAAATAAATATAAACACTTTAAAAGGGACAGCAAAGATACAGGGAAAAGAATTGATTTAAGCAAAAACTTCCGC
AGCCGGTCTGATGTTTTAGACAGTACAAACTTTCTGTTCAAACAGCTGATGGGAGAAACTGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCGCAAAGCGAAGACACAACGACAGAAATGCTCCTTGTACACCTAG
ATCAACACGAAGCAGAAAGTGATGAAGAACGAGAAGAACTGGAGACCGTACAATTTGAGGCGAGGGTGATTGCAAGGAAA
ATAAAAGAATTGGTGGAACAGCCTTTTCAAGTGTATGATGCAAAACAAAAAATAACCCGCAACTTGCAGTATCGAGATAT
CGTGATTTTGCTAAGGTCTATGCCGTGGGCACCGCAAATGATGGAGGAGTTGAAGAAGCAGGGAATCCCTGTTTATGCGA
ATCTTTCCTCCGGCTATTTTGAAGCTACAGAGGTATCTGTCGTTCTTTCTTTGTTAAAAGTAATCGATAATCCATATCAA
GATATCCCGCTTGCAGCTGTCTTAAGGTCACCTATTGTTCATTTAGACGAAAATGAGCTGGCACTCATTAGAACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTAAAGGCATTTATGAGTGTGACGCATTCTGATCATCCGACATGCAAAAAACTGG
AGCAATTCTTCTTCCTGTTGCGTAAATGGCGTGATTTCTCCATGACTCATTCGGTAGCAGAATTAATTTGGGAAGTGTAC
CGTGATACACATTATCTGGATTATGTTGGCGGGATGCCAGGGGGGAAGCAGAGACAGGCGAATCTCCGTGCTCTTTATGA
TCGAGCCAAACAGTATGAGAAGGCTGCATTTAGAGGGCTGTTTCGATTTCTTCGGTTTATCGAACGAATGCAGGAGCGTG
GAGATGATCTTGGGGCGGCGAAAACGTTCAGTGAAACAGAGGACGTTGTCCGTATGATGACGATTCATAGCAGTAAAGGA
CTCGAATTCCCAGTTGTGTTTACCGCAGGGCTTGGCAGGAATTTTAATATGATGGACCTAAATCAGTCCTACTTGCTTGA
TAAAGAACTAGGCTTTGGCAGCAAATATATTCACCCTGAGCTGAGAATTAGTTATACGACATTACCGCTTGTAGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAGCTGAGGGTTCTATATGTGGCGCTTACAAGGGCTAAGGAAAAATTA
TTTTTGGTCGGTTCTGTCAAAAATCAGGTGAAAGCTTTAAGCAAATGGCAAAATGCCGCAAACGGAGAAGAGTGGCTCCT
CCCTGATTTTGAACGTTATCAAGCCAAAACATACTTAGATTTTATTGGACCTGCCCTCATTCGTCACCAAGCAATGAAGC
CTATTTTAGAGGAATCAAGAGATGTCGTTCTTTCGCATCCATCATCATTTACCATCACATTTCATCAAGCCTCCGATCTT
CTAAAAGAGGATGTGTCACTTGAAAAGAAGAAGCAAGACGAAGTGATGCAAGCATTAATGGAAGGTGTGCCTGTTGAAGG
GTATGGCGATTATGAAGAGGAAGTAAAAGAAAGACTTTCATGGCGATATCCATATTTAGCGGCCTCTCAAGTCGGTACAA
AGCAGTCGGTTTCAGAGATAAAAAGGATGAAAGAAATCCAGGATGAATACAGTGTGCCTTCTTCTATTCGAAAACCGCGT
GCGACATTGTATGACAGGCCGGCTTTTATGAAGAAAAAAACACTCACTGCCGCAGAGCAAGGTACAGCGATGCATACGGT
GATGCAGCATATTCCGCTTCCTTCAGCTGAGGCCTATGATGAATCTCGTATACAAGAACTGCTCGATTCCTTAAAAGAGC
GAGACTTACTAACAGATGAGCAAATCGATTCCATCAATCGAGAAGGAATTGCTGCCTTCTTTTCTACTTCTATTGGTCAA
AAACTGCGGAAAGCGGACTGGGTGAAACGAGAAGTGTCGTTTAGTATGGTTTTACCTGTTAAAGAGGTATACAGCCACAT
TGAGGTAGAGGGTGAACCTGTGCTGATTCAAGGAATGATTGATTGTTTATTTGAAGCAGATGGTAAGCTCTATTTACTTG
ATTATAAAACGGATAGAGTGAGTGGCAGATTCTCAGGCGGACTAGAGGAAACGGAAGCGATGTTGAAAAAACGCTATGAA
ACACAAATAGCGTTGTATGCAAGAGCGGTAGAACGTTTAACAAGCAGAACATTAGAGGAGAAAATCCTCTATTTCTTTGA
TGGGAATTTAGAAATTTCTTTATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

65.804

100

0.66

Multiple sequence alignment