Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   GPA07_RS14530 Genome accession   NZ_CP046653
Coordinates   2783351..2786860 (-) Length   1169 a.a.
NCBI ID   WP_008355139.1    Uniprot ID   -
Organism   Bacillus sp. ms-22     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2778351..2791860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPA07_RS14520 (GPA07_14520) sbcD 2778467..2779642 (-) 1176 WP_008355143.1 exonuclease subunit SbcD -
  GPA07_RS14525 (GPA07_14525) addA 2779669..2783373 (-) 3705 WP_157292868.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  GPA07_RS14530 (GPA07_14530) addB 2783351..2786860 (-) 3510 WP_008355139.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  GPA07_RS14535 (GPA07_14535) - 2787028..2787474 (+) 447 WP_008355136.1 ferritin-like domain-containing protein -
  GPA07_RS14540 (GPA07_14540) - 2787670..2788080 (-) 411 WP_008355134.1 DoxX family protein -
  GPA07_RS14545 (GPA07_14545) - 2788138..2788977 (-) 840 WP_157292869.1 M56 family metallopeptidase -
  GPA07_RS14550 (GPA07_14550) - 2788970..2789392 (-) 423 WP_008355129.1 BlaI/MecI/CopY family transcriptional regulator -
  GPA07_RS14555 (GPA07_14555) - 2789664..2790809 (+) 1146 WP_008355127.1 AbrB family transcriptional regulator -
  GPA07_RS14560 (GPA07_14560) lepB 2790847..2791359 (-) 513 WP_008355125.1 signal peptidase I -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134862.97 Da        Isoelectric Point: 5.3128

>NTDB_id=406448 GPA07_RS14530 WP_008355139.1 2783351..2786860(-) (addB) [Bacillus sp. ms-22]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSDAGGMIRAKVFSFTRLAWSVLQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMMPEDIEKISVSSLHSEYTEERRAAE
KLHDLHVLYQQMEQYLQDEYVHSEDYLTLLAQQIPLSEELKGAHIYIDGFYQFTPQQLLVIEQLLLHAAKITAAFTVDRP
YHETQPNELDLFRMTGKTYFQLYQLAKECGASISEHMLERNKRHLHAPDLAYLENQYEQRPLQPYQEETPHLTVSKSANK
RAEIEGVARDILDLVREEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKDW
IVPPLFQLQKRLTKAQKVRDMVEALYVFLEEIQVPDKLEKARLEAEEAGRQAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFVGSMDLSRMYQVKCTFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSRHLYLSYSASDTEGRSLLPSTLIKYCQELMPHHQHALYVLDPEQLDDQEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNDLMTSPNRDVSKKIMSSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKEWKNLTKEDCVSYSRHAIEQLAPRLQKEILLSSN
RHAYMKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGMEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEEHFHILTKHVRRTFEEAGEKITNGEVEINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLTSEKDDVMIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=406448 GPA07_RS14530 WP_008355139.1 2783351..2786860(-) (addB) [Bacillus sp. ms-22]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAAGAGATCAAAGAACAACTTAG
GCTTGATCCGCTGGGTCCGCCCATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCAAAAA
CGTCTGACGCTGGCGGTATGATCAGAGCCAAAGTCTTTAGTTTTACTCGTCTTGCTTGGTCTGTTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTCACTAGCACGGGAATTCAGATGCTCTTAAGAAAGGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGATTTGTTGAGCAAATAGAAAAGACGATGGCTGAATTTAAAAGGTACT
GCATGATGCCGGAGGATATTGAAAAAATATCAGTATCCAGCTTGCATTCAGAGTATACAGAAGAAAGACGGGCTGCCGAA
AAGCTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGTATTTACAAGATGAATATGTGCATTCAGAGGACTACTT
GACACTTCTGGCACAGCAAATTCCATTATCTGAGGAATTAAAAGGAGCACACATTTATATAGATGGTTTTTACCAGTTTA
CCCCGCAGCAGCTCCTAGTCATTGAACAGCTTCTGCTCCATGCAGCAAAAATAACGGCTGCTTTCACAGTGGATCGTCCA
TATCATGAGACACAGCCAAATGAACTTGATTTATTTCGGATGACCGGTAAAACATATTTTCAGCTTTATCAGCTTGCCAA
AGAGTGTGGAGCGTCCATTTCTGAACACATGTTGGAGAGAAATAAGCGGCATCTTCATGCACCAGATCTTGCTTATTTAG
AAAACCAGTATGAACAAAGACCGCTTCAGCCTTATCAAGAAGAAACGCCTCATCTCACCGTGTCAAAAAGCGCAAACAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATTCTTGATTTAGTGAGAGAGGAAGGGCTTAGGCTGCGTGACATCTCAAT
TGTGGCAAGGCATGTTGACGACTATAAAGATACGTTAAAAGAGGTGTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCAATGCAGTATCATCCGCTCATTGAATTGATTCGTTCAAGCCTCGATGTCATAAAAGGGAACTGGCGGTAT
GAAGCGGTGTTTCGCTGTGTGAAAACAGAGTTCCTATTTCCGCTCGAACTCGCCAAAAACAAAGCAAGAGAACAGGCAGA
TCAGCTAGAAAACTATTGTATTGCTTATGGTGTAAAGGGAGAACGCTGGACAAACGGCTCTCGTTTTCATTACAGGCGAT
TTCAATCATTAGATGAAGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAAATGCTAAATGACGTAAAAGACTGG
ATTGTTCCTCCTCTTTTTCAGTTGCAAAAAAGATTGACAAAAGCGCAAAAGGTGAGAGACATGGTGGAGGCTCTCTATGT
CTTTTTAGAAGAAATACAAGTACCGGATAAGCTTGAAAAGGCAAGACTTGAGGCTGAAGAAGCAGGCAGGCAGGCTGAAG
CGATGCAGCACGGGCAAGTATGGGATGCTGTGATTCAATTAATGGATGAATTTGTTGAAATGCTCGGCGAGGAGGAGCTT
TCCTTTCCATTATTCCAACAGATGATGGATACAGGATTGGCATCTCTAAAGTTCGCTTTGATTCCACCTTCACTTGATCA
AGTATTTGTAGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGCACATTTATCATTGGCGTAAATGATGGCGTAA
TCCCTGCGCGGCCTTCTGATGAAAGTGTATTGTCTGAGGATGACCGGGAATGGTTAAAGCGTGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTGGATGAGCAGTTTTTAATTTACCAGGCGTTATCCAGCCCATCCCGCCATCTCTATTT
ATCCTATTCAGCTTCAGATACGGAAGGGCGATCTCTTTTGCCTTCGACGCTCATCAAGTATTGCCAAGAACTGATGCCAC
ATCACCAGCATGCGCTTTATGTGTTAGATCCAGAGCAACTGGATGATCAGGAACAATTAAAATTTGTAGCAAACGAGCAT
GTCTCGTTATCCTATACCGTCTCACAGCTGCAGCAATGGCTTAATCAATATCCCATCAGCGGTGTATGGTGGAGTGTATA
TAATGATTTAATGACGTCGCCTAATCGTGATGTATCAAAAAAGATCATGTCAAGTTTGTTTTTCACAAATAGAGCGAAGC
GATTAAAACCAAACGTCACAAAAGAGCTTTATGGTGATCATATTCAAGGCAGTGTTTCGAGAATGGAGAAATTCAATGCG
TGTGCATTTTCCCATTTTGCTTCACACGGGTTAAAACTAAAAGATCGCCAATTTTACAAATTGGAAGCACCCGATATCGG
TCAGCTGTTTCACTCGGCGTTAAAGCATATTTCAGATACACTTGTTGAACAAAAAAAGGAATGGAAAAACTTAACGAAGG
AAGATTGTGTGTCCTACTCAAGACATGCAATTGAACAGCTGGCTCCGCGTTTGCAAAAGGAAATTCTATTAAGCTCGAAC
CGGCATGCTTATATGAAAGAAAAACTTCAAAAAATCCTCATAAGAGTTTCTTCTATATTAAGCGAACATGCCAAAGTGAG
CGGATTCTCTCCGGTTGGACTTGAATTAGGATTTGGAGGTCAAGGCCCTCTGCCGCCATTTACTTTTCAATTAAAAAATG
GCTGCACGATGGAGCTGGTCGGAAGGATTGACCGAGTGGATAAAGCAGAAGGTTCAAAAGGACTGCTTTTAAGAATTGTC
GACTATAAGTCTAGCGAAAAGGGGCTGGATCTAGCAGAAGTATACTATGGTTTGGCGCTGCAGATGCTCACTTACTTAGA
CCTGACCATTACGTATTCTAAAGAGTGGCTAGGCATGGAGGCGACACCGGCAGGAATTTTATACTTCCATATTCATGATC
CTCTCATTCAAGCGCCTATTCCGCTGGCAGAGGATGAAATTGAACAGGAAATCTTCAAGAAATTTAAAATGAAAGGTCTA
TTGCTCGAAGACGTAGAAGCTGTGAAGCTGATGGATCAAACGCTTGAATCAGGAAGGTCGCAAGTCATCCAAGCCGGTTT
GAAAAAGGATGGGTCTTTCCGGTCTGACTCTGCGGTATTAAGTGAAGAGCATTTTCACATTCTCACAAAACACGTTCGGC
GTACTTTTGAAGAGGCAGGGGAGAAAATTACAAATGGTGAGGTAGAGATCAATCCATATAAATTGAAGGATCAAACACCT
TGCCGCTTTTGCTCCTTTAAGTCTATTTGTCAGTTTGATGAATCAATAGAGGATAATGAATTTAGGGTGCTTACATCTGA
AAAGGATGATGTCATGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.925

99.316

0.665

Multiple sequence alignment