Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GPA07_RS00480 Genome accession   NZ_CP046653
Coordinates   77503..78882 (-) Length   459 a.a.
NCBI ID   WP_071168236.1    Uniprot ID   A0AAC9NBF5
Organism   Bacillus sp. ms-22     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 72503..83882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPA07_RS00460 (GPA07_00460) ispF 73983..74459 (-) 477 WP_007496273.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GPA07_RS00465 (GPA07_00465) ispD 74463..75152 (-) 690 WP_008356486.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GPA07_RS00470 (GPA07_00470) - 75166..76266 (-) 1101 WP_157291864.1 PIN/TRAM domain-containing protein -
  GPA07_RS00475 (GPA07_00475) disA 76421..77500 (-) 1080 WP_008356482.1 DNA integrity scanning diadenylate cyclase DisA -
  GPA07_RS00480 (GPA07_00480) radA 77503..78882 (-) 1380 WP_071168236.1 DNA repair protein RadA Machinery gene
  GPA07_RS00485 (GPA07_00485) clpC 78976..81411 (-) 2436 WP_008356478.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  GPA07_RS00490 (GPA07_00490) - 81408..82499 (-) 1092 WP_008356475.1 protein arginine kinase -
  GPA07_RS00495 (GPA07_00495) - 82504..83061 (-) 558 WP_034738425.1 UvrB/UvrC motif-containing protein -
  GPA07_RS00500 (GPA07_00500) - 83076..83540 (-) 465 WP_008356472.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49979.46 Da        Isoelectric Point: 7.3829

>NTDB_id=406398 GPA07_RS00480 WP_071168236.1 77503..78882(-) (radA) [Bacillus sp. ms-22]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIESASLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLSEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGA

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=406398 GPA07_RS00480 WP_071168236.1 77503..78882(-) (radA) [Bacillus sp. ms-22]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGTAAAGAGCCGGCAAACCGTCGAAGTGCTTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCCATTGAAACATCCGAAGAACCCCGAATTAAAACGAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGCTCTCTCGTTCTTATTGGCGGTGATCCTGGGATTGGAAAGTCCACACT
ATTATTACAAGTTTCAGCACAGCTCTCAGACAAGAATCAGAATGTGTTATACATATCTGGTGAGGAATCGATTAAACAAA
CGAAGCTTAGAGCGGACCGCCTTGGCATTGAAAGTGCCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAAGAGATGAAACCCGCTTTTGTGGTGGTGGATTCAATTCAAACCGTTTATCAAAGCGATATTACGTCAGC
ACCTGGATCTGTTTCTCAGGTGAGGGAATGCACAGCACAGCTGATGAAAATTGCTAAGACGAATGGTATTCCGATTTTTA
TCGTTGGTCACGTGACCAAAGAAGGCTCGATTGCAGGCCCGCGCCTGTTAGAGCATATGGTGGATACGGTTCTTTATTTT
GAAGGGGAGCGGCACCATACGTTTCGCATTTTACGAGCGGTAAAAAACCGTTTTGGTTCAACGAATGAATTAGGGATTTT
TGAAATGAGAGAGGAAGGGCTCTCAGAAGTTTTAAACCCATCAGAAATCTTCTTAGAAGAGCGGTCAGCAGGTGTATCTG
GATCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCCGTTCTTGTGGAAATACAAGCATTAATTTCGCCGACAAGCTTT
GGAAACCCGCGGAGAATGGCCACAGGCCTTGATCATAATCGTGTGTCATTGCTCATGGCTGTGTTAGAAAAACGTGTAGG
GCTGCTTCTGCAAAATCAAGATGCATATTTAAAGGTCGCAGGTGGTGTAAAGCTTGACGAACCAGCGATTGATTTGGCCA
TTGCCGTCAGTATTGCATCAAGCTTTAAAGACGCAGCGCCGCACCAAGCGGATTGCTTTATAGGAGAAGTTGGTCTGACG
GGAGAAGTCAGAAGAGTTTCAAGAATTGAACAGCGTGTGCAGGAAGCTGCTAAACTAGGATTTAAGCGAATGTTTATTCC
TCAGGCGAATATAGATGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTCGGTGTAGAAAATGTAGCGGAGGCACTTCGTA
TTTCACTAGGGGGAGCATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.118

99.782

0.869
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608

Multiple sequence alignment