Detailed information NTG0406391

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GPU01_RS21305
• Genome accession: NZ_CP046645
• Coordinates: 4402943..4403743 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain EGKP3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4397943..4408743

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GPU01_RS21270 (GPU01_20950)	gspH	4398099..4398614 (+)	516	WP_004194939.1	type II secretion system minor pseudopilin GspH	-
GPU01_RS21275 (GPU01_20955)	gspI	4398611..4398976 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
GPU01_RS21280 (GPU01_20960)	gspJ	4398973..4399569 (+)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
GPU01_RS21285 (GPU01_20965)	gspK	4399566..4400552 (+)	987	WP_004894165.1	type II secretion system minor pseudopilin GspK	-
GPU01_RS21290 (GPU01_20970)	gspL	4400539..4401732 (+)	1194	WP_004894169.1	type II secretion system protein GspL	-
GPU01_RS21295 (GPU01_20975)	-	4401725..4402210 (+)	486	WP_004183037.1	type II secretion system protein M	-
GPU01_RS21300 (GPU01_20980)	-	4402200..4402946 (+)	747	WP_004145897.1	type II secretion system protein N	-
GPU01_RS21305 (GPU01_20985)	pilD	4402943..4403743 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
GPU01_RS21310 (GPU01_20990)	hrpB	4403791..4406220 (-)	2430	WP_004894175.1	ATP-dependent helicase HrpB	-
GPU01_RS21315 (GPU01_20995)	thpR	4406293..4406829 (+)	537	WP_004894180.1	RNA 2',3'-cyclic phosphodiesterase	-
GPU01_RS21320 (GPU01_21000)	sfsA	4406829..4407545 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
GPU01_RS21325 (GPU01_21005)	dksA	4407708..4408163 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=406391 GPU01_RS21305 WP_002888947.1 4402943..4403743(+) (pilD) [Klebsiella pneumoniae strain EGKP3]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=406391 GPU01_RS21305 WP_002888947.1 4402943..4403743(+) (pilD) [Klebsiella pneumoniae strain EGKP3]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGAGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425