Detailed information NTG0406045

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GPJ73_RS22265
• Genome accession: NZ_CP046612
• Coordinates: 4545496..4546296 (+)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain WCGKP294
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4540496..4551296

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GPJ73_RS22230 (GPJ73_22230)	gspH	4540652..4541167 (+)	516	WP_032414529.1	type II secretion system minor pseudopilin GspH	-
GPJ73_RS22235 (GPJ73_22235)	gspI	4541164..4541529 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
GPJ73_RS22240 (GPJ73_22240)	gspJ	4541526..4542122 (+)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
GPJ73_RS22245 (GPJ73_22245)	gspK	4542119..4543105 (+)	987	WP_077254116.1	type II secretion system minor pseudopilin GspK	-
GPJ73_RS22250 (GPJ73_22250)	gspL	4543092..4544285 (+)	1194	WP_032434208.1	type II secretion system protein GspL	-
GPJ73_RS22255 (GPJ73_22255)	-	4544278..4544763 (+)	486	WP_004145896.1	type II secretion system protein M	-
GPJ73_RS22260 (GPJ73_22260)	-	4544753..4545499 (+)	747	WP_032434210.1	type II secretion system protein N	-
GPJ73_RS22265 (GPJ73_22265)	pilD	4545496..4546296 (+)	801	WP_004213441.1	prepilin peptidase	Machinery gene
GPJ73_RS22270 (GPJ73_22270)	hrpB	4546344..4548773 (-)	2430	WP_032434212.1	ATP-dependent helicase HrpB	-
GPJ73_RS22275 (GPJ73_22275)	thpR	4548846..4549382 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
GPJ73_RS22280 (GPJ73_22280)	sfsA	4549382..4550098 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
GPJ73_RS22285 (GPJ73_22285)	dksA	4550261..4550716 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=406045 GPJ73_RS22265 WP_004213441.1 4545496..4546296(+) (pilD) [Klebsiella pneumoniae strain WCGKP294]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=406045 GPJ73_RS22265 WP_004213441.1 4545496..4546296(+) (pilD) [Klebsiella pneumoniae strain WCGKP294]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGAGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425