Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GO005_RS02345 Genome accession   NZ_CP046592
Coordinates   426439..426888 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain TR21     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 421439..431888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO005_RS02310 (GO005_02295) yckA 421714..422394 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  GO005_RS02315 (GO005_02300) yckB 422404..423267 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  GO005_RS02320 (GO005_02305) - 423429..423621 (+) 193 Protein_416 hypothetical protein -
  GO005_RS02325 (GO005_02310) - 423657..423977 (+) 321 Protein_417 RDD family protein -
  GO005_RS02330 (GO005_02315) yckD 424059..424391 (+) 333 WP_015252849.1 YckD family protein -
  GO005_RS02335 (GO005_02320) bglC 424544..425977 (+) 1434 WP_032722901.1 6-phospho-beta-glucosidase -
  GO005_RS02340 (GO005_02325) nin/comJ 426014..426412 (-) 399 WP_032722902.1 DNA-entry nuclease inhibitor Regulator
  GO005_RS02345 (GO005_02330) nucA/comI 426439..426888 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  GO005_RS02350 (GO005_02335) tlpC 427056..428777 (-) 1722 WP_029727077.1 methyl-accepting chemotaxis protein TlpC -
  GO005_RS02355 (GO005_02340) hxlB 428888..429445 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  GO005_RS02360 (GO005_02345) hxlA 429451..430083 (-) 633 WP_032722903.1 3-hexulose-6-phosphate synthase -
  GO005_RS02365 (GO005_02350) hxlR 430316..430678 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=405844 GO005_RS02345 WP_003234608.1 426439..426888(-) (nucA/comI) [Bacillus subtilis strain TR21]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=405844 GO005_RS02345 WP_003234608.1 426439..426888(-) (nucA/comI) [Bacillus subtilis strain TR21]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment