Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   GO004_RS15910 Genome accession   NZ_CP046591
Coordinates   3054309..3055454 (-) Length   381 a.a.
NCBI ID   WP_015714890.1    Uniprot ID   -
Organism   Bacillus subtilis strain R31     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3049309..3060454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO004_RS15875 (GO004_15865) - 3049862..3050422 (-) 561 WP_015714897.1 hypothetical protein -
  GO004_RS15880 (GO004_15870) - 3050415..3051062 (-) 648 WP_015714896.1 ABC transporter ATP-binding protein -
  GO004_RS15885 (GO004_15875) - 3051065..3051862 (-) 798 WP_231573542.1 hypothetical protein -
  GO004_RS15890 (GO004_15880) - 3051872..3052546 (-) 675 WP_015714894.1 hypothetical protein -
  GO004_RS15895 (GO004_15885) - 3052533..3053321 (-) 789 WP_015714893.1 membrane protein -
  GO004_RS15900 (GO004_15890) - 3053470..3053949 (-) 480 WP_015714892.1 hypothetical protein -
  GO004_RS15905 (GO004_15895) phrF 3054206..3054325 (-) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  GO004_RS15910 (GO004_15900) rapF 3054309..3055454 (-) 1146 WP_015714890.1 response regulator aspartate phosphatase RapF Regulator
  GO004_RS21970 (GO004_15905) - 3055457..3055687 (+) 231 WP_015714889.1 hypothetical protein -
  GO004_RS15915 (GO004_15910) ywhK 3055684..3057039 (+) 1356 WP_195727704.1 YncE family protein -
  GO004_RS15920 (GO004_15915) ywhL 3057078..3058454 (+) 1377 WP_003244415.1 YncE family protein -
  GO004_RS15925 (GO004_15920) albG 3058460..3059161 (-) 702 WP_195727705.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  GO004_RS15930 (GO004_15925) albF 3059158..3060438 (-) 1281 WP_003242974.1 pitrilysin family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45571.01 Da        Isoelectric Point: 4.9848

>NTDB_id=405815 GO004_RS15910 WP_015714890.1 3054309..3055454(-) (rapF) [Bacillus subtilis strain R31]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLNLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=405815 GO004_RS15910 WP_015714890.1 3054309..3055454(-) (rapF) [Bacillus subtilis strain R31]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTAATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAAAATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCGTCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGAAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATAACTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(223-290)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

98.688

100

0.987
  rapC Bacillus subtilis subsp. subtilis str. 168

57.368

99.738

0.572

Multiple sequence alignment