Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GO004_RS12080 Genome accession   NZ_CP046591
Coordinates   2313009..2313458 (+) Length   149 a.a.
NCBI ID   WP_157678489.1    Uniprot ID   -
Organism   Bacillus subtilis strain R31     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2308009..2318458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO004_RS12060 (GO004_12050) hxlR 2309219..2309581 (-) 363 WP_003246265.1 transcriptional activator HxlR -
  GO004_RS12065 (GO004_12055) hxlA 2309814..2310446 (+) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  GO004_RS12070 (GO004_12060) hxlB 2310452..2311009 (+) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  GO004_RS12075 (GO004_12065) tlpC 2311120..2312841 (+) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  GO004_RS12080 (GO004_12070) nucA/comI 2313009..2313458 (+) 450 WP_157678489.1 DNA-entry nuclease Machinery gene
  GO004_RS12085 (GO004_12075) nin/comJ 2313485..2313883 (+) 399 WP_032722902.1 DNA-entry nuclease inhibitor Regulator
  GO004_RS12090 (GO004_12080) bglC 2313920..2315353 (-) 1434 WP_032722901.1 6-phospho-beta-glucosidase -
  GO004_RS12095 (GO004_12085) yckD 2315506..2315838 (-) 333 WP_015252849.1 YckD family protein -
  GO004_RS12100 (GO004_12090) - 2315920..2316240 (-) 321 Protein_2400 RDD family protein -
  GO004_RS12105 (GO004_12095) - 2316276..2316468 (-) 193 Protein_2401 hypothetical protein -
  GO004_RS12110 (GO004_12100) yckB 2316630..2317493 (+) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  GO004_RS12115 (GO004_12105) yckA 2317503..2318183 (+) 681 WP_003234624.1 amino acid ABC transporter permease -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16458.43 Da        Isoelectric Point: 4.5282

>NTDB_id=405803 GO004_RS12080 WP_157678489.1 2313009..2313458(+) (nucA/comI) [Bacillus subtilis strain R31]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=405803 GO004_RS12080 WP_157678489.1 2313009..2313458(+) (nucA/comI) [Bacillus subtilis strain R31]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

99.329

100

0.993

Multiple sequence alignment