Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GNK04_RS00585 Genome accession   NZ_CP046564
Coordinates   113362..114735 (+) Length   457 a.a.
NCBI ID   WP_159780805.1    Uniprot ID   A0A7Z2Q9Y7
Organism   Bacillus sp. N1-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 108362..119735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNK04_RS00565 (GNK04_00565) - 108540..109001 (+) 462 WP_159780801.1 CtsR family transcriptional regulator -
  GNK04_RS00570 (GNK04_00570) - 109039..109575 (+) 537 WP_159780802.1 UvrB/UvrC motif-containing protein -
  GNK04_RS00575 (GNK04_00575) - 109575..110672 (+) 1098 WP_159780803.1 protein arginine kinase -
  GNK04_RS00580 (GNK04_00580) clpC 110669..113110 (+) 2442 WP_159780804.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  GNK04_RS00585 (GNK04_00585) radA 113362..114735 (+) 1374 WP_159780805.1 DNA repair protein RadA Machinery gene
  GNK04_RS00590 (GNK04_00590) disA 114739..115800 (+) 1062 WP_159780806.1 DNA integrity scanning diadenylate cyclase DisA -
  GNK04_RS00595 (GNK04_00595) - 115961..117058 (+) 1098 WP_159780807.1 PIN/TRAM domain-containing protein -
  GNK04_RS00600 (GNK04_00600) ispD 117084..117776 (+) 693 WP_159780808.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GNK04_RS00605 (GNK04_00605) ispF 117776..118249 (+) 474 WP_159780809.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49974.38 Da        Isoelectric Point: 6.3279

>NTDB_id=405529 GNK04_RS00585 WP_159780805.1 113362..114735(+) (radA) [Bacillus sp. N1-1]
MAKKKTKFMCQDCGYESPKWMGKCPGCHQWNTMVEEMVPGENDRRRFVTSSNETASKPTSIRSIQKEMEPRILTHITELD
RVLGGGVVPGSLVLVGGDPGIGKSTLLLQTSSKLADQDHPVLYISGEESVKQTKLRADRLGVDAEKLFVLAETDLEYISK
AIEEINPQLVIIDSIQTIFRSEVTSAPGSVSQVRECTSQLMRIAKTKGIAVFIVGHVTKEGSIAGPRLLEHMVDAVLYFE
GERHHTFRILRGVKNRFGSTNEIGVFEMKEEGLEEVLNPSEIFLEERSSGSAGSTVVASMEGTRTVLVEIQALISPTSFG
NPRRTATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLNVAGGVRLDEPAIDLAIAVSIASSFRDKPTRPSDIVVGEIGLTG
EIRRVSRIEQRVIEAAKLGFERAIIPKKNIGGWTFPEGIQVIGVQTVDEALKEALGG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=405529 GNK04_RS00585 WP_159780805.1 113362..114735(+) (radA) [Bacillus sp. N1-1]
ATGGCGAAGAAAAAGACGAAATTTATGTGTCAGGATTGCGGCTATGAATCTCCAAAATGGATGGGAAAATGTCCAGGATG
TCATCAATGGAACACGATGGTAGAAGAAATGGTTCCAGGAGAAAACGATCGTAGACGATTCGTTACTTCAAGCAATGAAA
CGGCGAGTAAACCCACATCTATTAGAAGTATCCAAAAAGAAATGGAGCCTAGAATACTAACCCACATCACGGAACTTGAT
CGTGTATTAGGCGGTGGTGTCGTTCCGGGTTCACTCGTTCTTGTTGGGGGTGATCCGGGGATTGGTAAATCAACGCTCTT
ATTACAAACGTCGTCGAAGTTGGCTGATCAGGATCACCCCGTTCTGTATATCTCTGGTGAGGAGTCGGTCAAGCAAACGA
AGCTTCGTGCAGATCGTTTAGGTGTTGATGCGGAAAAATTATTTGTATTAGCGGAGACGGATCTTGAGTATATTAGTAAA
GCGATTGAAGAAATAAACCCGCAACTTGTCATTATCGACTCAATCCAAACGATTTTCAGATCAGAAGTTACATCTGCTCC
GGGTAGTGTTTCTCAAGTTCGTGAATGTACTTCCCAACTTATGCGTATAGCTAAAACAAAGGGAATTGCAGTTTTTATTG
TCGGGCATGTAACGAAAGAAGGATCAATTGCGGGTCCGAGACTTTTAGAACATATGGTAGATGCGGTGCTTTACTTTGAG
GGAGAGCGTCACCATACATTCCGAATTCTTCGCGGCGTAAAAAACCGTTTTGGATCGACAAATGAGATAGGTGTTTTTGA
GATGAAGGAAGAGGGTCTAGAAGAAGTGCTTAATCCTTCTGAGATTTTTCTAGAAGAACGATCTTCTGGTTCTGCAGGGT
CAACGGTTGTTGCATCTATGGAGGGAACGCGAACGGTCCTTGTCGAAATACAAGCTCTTATATCACCTACTAGCTTTGGA
AATCCACGTAGAACGGCAACAGGTATTGATCATAACCGAGTATCGTTATTAATGGCGGTTCTTGAGAAAAGAGTAGGCCT
TCTTTTGCAAAATCAGGATGCTTATTTAAATGTGGCTGGTGGTGTAAGACTTGATGAGCCTGCAATTGACCTTGCTATTG
CTGTTTCAATTGCTTCAAGTTTTAGAGACAAACCAACACGTCCTTCTGACATAGTAGTGGGTGAAATTGGATTGACCGGA
GAAATTAGACGAGTATCTCGTATTGAACAACGAGTCATCGAAGCCGCTAAACTTGGTTTTGAACGGGCTATTATTCCAAA
GAAGAACATAGGTGGATGGACTTTTCCAGAAGGCATTCAGGTAATCGGTGTTCAAACAGTAGATGAAGCATTAAAAGAAG
CACTAGGAGGGTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Subunit ChlI of Mg-chelatase (ChlI)

(349-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2Q9Y7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.109

100

0.753
  radA Streptococcus pneumoniae Rx1

61.623

99.781

0.615
  radA Streptococcus pneumoniae D39

61.623

99.781

0.615
  radA Streptococcus pneumoniae R6

61.623

99.781

0.615
  radA Streptococcus pneumoniae TIGR4

61.623

99.781

0.615
  radA Streptococcus mitis NCTC 12261

61.623

99.781

0.615
  radA Streptococcus mitis SK321

61.623

99.781

0.615

Multiple sequence alignment