Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GM698_RS02660 Genome accession   NZ_CP046532
Coordinates   572336..572890 (-) Length   184 a.a.
NCBI ID   WP_209289626.1    Uniprot ID   -
Organism   Mannheimia sp. ZY171111     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 567336..577890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM698_RS02645 (GM698_02620) - 568575..569138 (+) 564 WP_209289624.1 nitroreductase family protein -
  GM698_RS02650 (GM698_02625) - 569140..569961 (+) 822 WP_176812269.1 SDR family NAD(P)-dependent oxidoreductase -
  GM698_RS02655 (GM698_02630) - 570002..572335 (-) 2334 WP_209289625.1 Tex family protein -
  GM698_RS02660 (GM698_02635) pilF 572336..572890 (-) 555 WP_209289626.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GM698_RS02665 (GM698_02640) - 573047..574207 (-) 1161 WP_159629169.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  GM698_RS02670 (GM698_02645) - 574461..575360 (+) 900 WP_176807953.1 metal ABC transporter substrate-binding protein -
  GM698_RS02675 (GM698_02650) - 575368..576111 (+) 744 WP_176807952.1 metal ABC transporter ATP-binding protein -
  GM698_RS02680 (GM698_02655) - 576185..577009 (-) 825 WP_176807951.1 metal ABC transporter permease -
  GM698_RS02685 (GM698_02660) - 577002..577862 (-) 861 WP_159629165.1 metal ABC transporter permease -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 21104.06 Da        Isoelectric Point: 8.4305

>NTDB_id=405176 GM698_RS02660 WP_209289626.1 572336..572890(-) (pilF) [Mannheimia sp. ZY171111]
MFAKFFSKITACIILFLLAACSSRFHPSSDAEFNRSEAVKARINLALAYLEQSNFPKAKQNIDKALIHDEKDYLPYSVLA
YYYQKTGDTENAEKSYQTALNLSKNRPDVLNNYGTFLCKQGQFQQAYHQFEQAVESEAPYYHQADTLENIILCAKKEPNL
NKITETLTKLEKLDKQRAILLKGK

Nucleotide


Download         Length: 555 bp        

>NTDB_id=405176 GM698_RS02660 WP_209289626.1 572336..572890(-) (pilF) [Mannheimia sp. ZY171111]
ATGTTTGCAAAATTTTTCTCAAAAATAACCGCTTGTATAATACTATTTTTATTAGCAGCTTGTTCTTCTCGCTTTCATCC
TTCCTCTGACGCAGAATTTAATCGATCGGAAGCGGTGAAAGCTCGCATTAATCTTGCTTTGGCTTATTTAGAGCAGAGCA
ATTTCCCCAAAGCGAAACAAAATATTGATAAAGCCCTGATTCACGATGAAAAGGATTATCTACCTTATTCGGTATTGGCG
TACTATTATCAAAAAACAGGGGATACTGAAAATGCTGAAAAATCCTATCAAACTGCCCTAAATTTAAGTAAAAATCGACC
TGATGTTTTAAATAATTACGGTACTTTTTTATGTAAGCAAGGTCAATTTCAGCAAGCTTATCATCAATTTGAACAAGCAG
TTGAAAGCGAAGCACCTTATTATCATCAGGCAGATACTTTAGAAAACATCATTTTATGTGCGAAAAAAGAGCCGAATCTG
AATAAAATAACGGAAACCTTAACTAAGCTCGAAAAATTAGACAAACAACGAGCAATTTTATTGAAAGGAAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(96-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

62.5

100

0.625
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.953

92.935

0.446

Multiple sequence alignment