Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   GOM47_RS09475 Genome accession   NZ_CP046524
Coordinates   1911560..1912858 (-) Length   432 a.a.
NCBI ID   WP_235080647.1    Uniprot ID   -
Organism   Streptococcus oralis strain SOT     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1889668..1929478 1911560..1912858 within 0


Gene organization within MGE regions


Location: 1889668..1929478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOM47_RS09345 (GOM47_09350) - 1889668..1890819 (-) 1152 WP_414930277.1 phage major capsid protein -
  GOM47_RS09350 (GOM47_09355) - 1890912..1891058 (-) 147 WP_001003898.1 DUF2292 domain-containing protein -
  GOM47_RS09355 (GOM47_09360) - 1891389..1892843 (-) 1455 WP_235080635.1 virulence-associated E family protein -
  GOM47_RS09360 (GOM47_09365) - 1892836..1893351 (-) 516 WP_084856430.1 hypothetical protein -
  GOM47_RS09365 (GOM47_09370) - 1893342..1893611 (-) 270 WP_084856428.1 hypothetical protein -
  GOM47_RS09370 (GOM47_09375) - 1893613..1893822 (-) 210 WP_084856425.1 hypothetical protein -
  GOM47_RS09375 (GOM47_09380) - 1893819..1894250 (-) 432 WP_084856423.1 hypothetical protein -
  GOM47_RS09380 - 1894483..1894632 (-) 150 WP_180383029.1 hypothetical protein -
  GOM47_RS09385 (GOM47_09385) - 1894636..1895064 (-) 429 WP_084856421.1 hypothetical protein -
  GOM47_RS09390 (GOM47_09390) - 1895183..1895431 (-) 249 WP_084856419.1 hypothetical protein -
  GOM47_RS09395 (GOM47_09395) - 1895446..1895646 (-) 201 WP_084856417.1 helix-turn-helix transcriptional regulator -
  GOM47_RS09400 (GOM47_09400) - 1895849..1896307 (+) 459 WP_084856416.1 helix-turn-helix domain-containing protein -
  GOM47_RS09405 (GOM47_09405) - 1896458..1897624 (+) 1167 WP_084856415.1 tyrosine-type recombinase/integrase -
  GOM47_RS09410 (GOM47_09410) - 1897781..1898509 (-) 729 WP_235080636.1 ABC transporter ATP-binding protein -
  GOM47_RS09415 (GOM47_09415) - 1898509..1899516 (-) 1008 WP_235080637.1 ABC transporter substrate-binding protein -
  GOM47_RS09420 (GOM47_09420) - 1899555..1900313 (-) 759 WP_235080638.1 ABC transporter permease -
  GOM47_RS09425 (GOM47_09425) - 1900276..1900566 (-) 291 WP_235080639.1 MTH1187 family thiamine-binding protein -
  GOM47_RS09430 (GOM47_09430) polA 1900843..1903476 (-) 2634 WP_235080640.1 DNA polymerase I -
  GOM47_RS09435 (GOM47_09435) - 1903561..1904670 (-) 1110 WP_235080641.1 SH3 domain-containing protein -
  GOM47_RS09440 (GOM47_09440) - 1904761..1905036 (-) 276 WP_235080642.1 Veg family protein -
  GOM47_RS09445 (GOM47_09445) dnaB 1905038..1906390 (-) 1353 WP_235080643.1 replicative DNA helicase -
  GOM47_RS09450 (GOM47_09450) rplI 1906434..1906886 (-) 453 WP_195215772.1 50S ribosomal protein L9 -
  GOM47_RS09455 (GOM47_09455) - 1906883..1908856 (-) 1974 WP_235080644.1 DHH family phosphoesterase -
  GOM47_RS09460 (GOM47_09460) - 1909013..1910191 (-) 1179 WP_235080645.1 acetyl-CoA C-acetyltransferase -
  GOM47_RS09465 (GOM47_09465) hpf 1910273..1910821 (-) 549 WP_000599113.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  GOM47_RS09470 (GOM47_09470) comFC/cflB 1910901..1911563 (-) 663 WP_235080646.1 ComF family protein Machinery gene
  GOM47_RS09475 (GOM47_09475) comFA/cflA 1911560..1912858 (-) 1299 WP_235080647.1 DEAD/DEAH box helicase Machinery gene
  GOM47_RS09480 (GOM47_09480) - 1912915..1913550 (+) 636 WP_235080648.1 YigZ family protein -
  GOM47_RS09485 (GOM47_09485) - 1913565..1914008 (+) 444 WP_235080649.1 PH domain-containing protein -
  GOM47_RS09490 (GOM47_09490) cysK 1914105..1915031 (+) 927 WP_235080650.1 cysteine synthase A -
  GOM47_RS09495 (GOM47_09495) tsf 1915146..1916186 (-) 1041 WP_084974703.1 translation elongation factor Ts -
  GOM47_RS09500 (GOM47_09500) rpsB 1916265..1917044 (-) 780 WP_235080651.1 30S ribosomal protein S2 -
  GOM47_RS09505 (GOM47_09505) pcsB 1917267..1918472 (-) 1206 WP_235080652.1 peptidoglycan hydrolase PcsB -
  GOM47_RS09510 (GOM47_09510) mreD 1918566..1919060 (-) 495 WP_235080653.1 rod shape-determining protein MreD -
  GOM47_RS09515 (GOM47_09515) mreC 1919063..1919878 (-) 816 WP_235080654.1 rod shape-determining protein MreC -
  GOM47_RS09520 (GOM47_09520) - 1919939..1920733 (-) 795 WP_235080655.1 energy-coupling factor transporter transmembrane component T family protein -
  GOM47_RS09525 (GOM47_09525) - 1920726..1921565 (-) 840 WP_235080656.1 energy-coupling factor transporter ATPase -
  GOM47_RS09530 (GOM47_09530) - 1921550..1922377 (-) 828 WP_235080657.1 energy-coupling factor ABC transporter ATP-binding protein -
  GOM47_RS09535 (GOM47_09535) pgsA 1922374..1922919 (-) 546 WP_235080658.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  GOM47_RS09540 (GOM47_09540) rodZ 1922930..1923754 (-) 825 WP_235080659.1 cytoskeleton protein RodZ -
  GOM47_RS09545 (GOM47_09545) yfmH 1923790..1925073 (-) 1284 WP_235080660.1 EF-P 5-aminopentanol modification-associated protein YfmH -
  GOM47_RS09550 (GOM47_09550) yfmF 1925070..1926320 (-) 1251 WP_235080661.1 EF-P 5-aminopentanol modification-associated protein YfmF -
  GOM47_RS09555 (GOM47_09555) yaaA 1926481..1926849 (+) 369 WP_195215749.1 S4 domain-containing protein YaaA -
  GOM47_RS09560 (GOM47_09560) recF 1926852..1927949 (+) 1098 WP_235080662.1 DNA replication/repair protein RecF -
  GOM47_RS09565 (GOM47_09565) guaB 1928000..1929478 (-) 1479 WP_235080663.1 IMP dehydrogenase -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49654.81 Da        Isoelectric Point: 9.0664

>NTDB_id=405022 GOM47_RS09475 WP_235080647.1 1911560..1912858(-) (comFA/cflA) [Streptococcus oralis strain SOT]
MKVNPNYLGRLFTEKEITEEERQVAVKLPAMRKEKGKLFCQRCNSSILEEWYLPIGAYYCRECLLMKRVRSDQALYYFPQ
EDFPKQDVLKWRGKLTPFQEKVSEGLIRAVEKKEPTLVHAVTGAGKTEMIYQVVAKVINNGGAVCLASPRIDVCLELYKR
LQNDFACEIALLHGESEPYFRTPLVVATTHQLLKFHHAFDLLIVDEVDAFPYVDNPILYYAVNQCVKEEGLKIFLTATST
DELDKKVRTGELKRLSLPRRFHGNPLIIPKLVWLSDFNRYIEKSQLSPKLKSYIKKQRRTSYPLLIFASEIKKGEKLKEL
LQEQFPNENIGFVSSITENRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGELLFFHDGLNVSIKKAIKEIKQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=405022 GOM47_RS09475 WP_235080647.1 1911560..1912858(-) (comFA/cflA) [Streptococcus oralis strain SOT]
ATGAAAGTAAATCCAAATTATCTCGGTCGTTTGTTTACTGAGAAAGAAATAACTGAAGAAGAACGACAGGTAGCAGTGAA
ACTGCCAGCAATGAGAAAAGAGAAGGGGAAACTGTTTTGTCAACGTTGTAATAGTTCGATTTTAGAAGAATGGTATTTGC
CTATTGGCGCATACTATTGTAGGGAGTGTTTGCTGATGAAGCGAGTCAGGAGTGATCAAGCCTTATACTATTTTCCGCAG
GAAGATTTTCCTAAACAAGATGTTCTTAAGTGGCGTGGTAAGTTAACCCCTTTTCAGGAGAAGGTGTCAGAGGGATTGAT
TCGAGCAGTCGAAAAGAAAGAACCGACCTTGGTTCATGCTGTAACAGGAGCTGGAAAGACGGAGATGATTTATCAAGTTG
TGGCCAAGGTAATCAATAATGGTGGTGCAGTGTGTTTGGCCAGTCCTCGAATTGATGTATGTTTGGAATTGTATAAGCGA
CTGCAGAATGACTTTGCTTGTGAGATAGCACTGCTTCATGGCGAATCAGAGCCCTATTTTCGAACTCCACTAGTTGTTGC
AACGACTCACCAGTTGTTAAAATTTCATCATGCTTTTGATTTGCTGATAGTAGATGAAGTAGATGCCTTTCCTTATGTTG
ACAACCCTATACTTTACTACGCTGTAAACCAATGTGTAAAGGAGGAGGGGTTAAAGATATTCCTTACAGCGACCTCTACA
GATGAGTTAGATAAGAAGGTTCGCACTGGAGAATTAAAACGATTGAGCTTGCCAAGACGATTTCATGGAAATCCATTGAT
TATTCCAAAGCTAGTTTGGTTATCAGATTTTAATCGCTATATAGAAAAGAGTCAGTTGTCTCCAAAGTTAAAGTCCTACA
TTAAGAAGCAGAGAAGAACAAGTTATCCGTTGTTAATCTTTGCATCTGAGATTAAGAAAGGCGAGAAACTAAAAGAACTC
TTGCAGGAACAGTTTCCAAATGAAAACATCGGCTTTGTGTCCTCTATCACAGAAAATCGATTAGAGCAGGTACAAGCTTT
TCGAGATGGAGAGTTGACAATCCTTATTAGTACAACAATTTTGGAGCGTGGGGTCACCTTTCCTTGTGTGGATGTTTTTG
TTGTAGAAGCTAATCATCGTCTCTTTACCAAGTCTAGCTTGATTCAGATTGGAGGGCGAGTTGGGCGCAGTATGGATAGA
CCGACTGGTGAACTGCTCTTCTTTCATGATGGATTAAATGTTTCGATCAAAAAAGCAATCAAGGAAATAAAGCAGATGAA
CAAGGAGGCAGGCTTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-396)

Type III restriction enzyme, res subunit (ResIII)

(94-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

89.815

100

0.898
  comFA/cflA Streptococcus pneumoniae Rx1

88.657

100

0.887
  comFA/cflA Streptococcus pneumoniae D39

88.657

100

0.887
  comFA/cflA Streptococcus pneumoniae R6

88.657

100

0.887
  comFA/cflA Streptococcus pneumoniae TIGR4

88.426

100

0.884
  comFA/cflA Streptococcus mitis SK321

88.426

100

0.884
  comFA Lactococcus lactis subsp. cremoris KW2

50.976

94.907

0.484
  comFA Latilactobacillus sakei subsp. sakei 23K

37.558

100

0.377

Multiple sequence alignment