Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HWQ50_RS30020 Genome accession   NZ_CP058331
Coordinates   6462649..6463272 (-) Length   207 a.a.
NCBI ID   WP_023110484.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ACR22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6457649..6468272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ50_RS30000 (HWQ50_29815) aroB 6458256..6459362 (-) 1107 WP_003116138.1 3-dehydroquinate synthase -
  HWQ50_RS30005 (HWQ50_29820) aroK 6459412..6459930 (-) 519 WP_003095833.1 shikimate kinase AroK -
  HWQ50_RS30010 (HWQ50_29825) pilQ 6459942..6462074 (-) 2133 WP_023110483.1 type 4a pilus secretin PilQ Machinery gene
  HWQ50_RS30015 (HWQ50_29830) comL/pilP 6462128..6462652 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  HWQ50_RS30020 (HWQ50_29835) pilO 6462649..6463272 (-) 624 WP_023110484.1 type 4a pilus biogenesis protein PilO Machinery gene
  HWQ50_RS30025 (HWQ50_29840) pilN 6463269..6463865 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  HWQ50_RS30030 (HWQ50_29845) pilM 6463865..6464929 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  HWQ50_RS30035 (HWQ50_29850) - 6465114..6467585 (+) 2472 WP_179118826.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 23027.46 Da        Isoelectric Point: 5.0913

>NTDB_id=404983 HWQ50_RS30020 WP_023110484.1 6462649..6463272(-) (pilO) [Pseudomonas aeruginosa strain ACR22]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMRILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=404983 HWQ50_RS30020 WP_023110484.1 6462649..6463272(-) (pilO) [Pseudomonas aeruginosa strain ACR22]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAAGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGGATCCTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.263

91.787

0.415
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396

Multiple sequence alignment