Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   GE024_RS08125 Genome accession   NZ_CP046521
Coordinates   1623549..1624874 (-) Length   441 a.a.
NCBI ID   WP_093999505.1    Uniprot ID   A0A3P5Y1B1
Organism   Streptococcus canis strain HL_100     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1618549..1629874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GE024_RS08110 (GE024_08150) - 1621281..1622232 (+) 952 Protein_1544 IS30 family transposase -
  GE024_RS08115 (GE024_08155) raiA 1622284..1622832 (-) 549 WP_093999503.1 ribosome-associated translation inhibitor RaiA -
  GE024_RS11225 - 1622912..1623334 (-) 423 WP_093999504.1 ComF family protein -
  GE024_RS08125 (GE024_08165) comFA/cflA 1623549..1624874 (-) 1326 WP_093999505.1 DEAD/DEAH box helicase Machinery gene
  GE024_RS08130 (GE024_08170) - 1624930..1625562 (+) 633 WP_093999506.1 YigZ family protein -
  GE024_RS08135 (GE024_08175) cysK 1625680..1626615 (+) 936 WP_003044700.1 cysteine synthase A -
  GE024_RS08140 (GE024_08180) tnpA 1626888..1627352 (-) 465 Protein_1550 IS200/IS605 family transposase -
  GE024_RS08145 (GE024_08185) - 1627540..1627899 (-) 360 WP_093998981.1 S1 RNA-binding domain-containing protein -
  GE024_RS08150 (GE024_08190) - 1627899..1629299 (-) 1401 WP_093998980.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49753.89 Da        Isoelectric Point: 9.7993

>NTDB_id=404866 GE024_RS08125 WP_093999505.1 1623549..1624874(-) (comFA/cflA) [Streptococcus canis strain HL_100]
MEGIENYYGRLFLENQLPEEGKHLAKPLESIVITKGKVTCQRCRYHISEEERLPSGAYYCRFCLVFGRNQSDKSLYYMSP
KPFPQGSCLKWKGQLTPHQKKISQQLVRNVQAKKPTLVHAVTGAGKTEMIYAAIARVIEAGGWVCLASPRVDVCIEVAKR
LSQAFSCQVCLMHAGSSPYQRSPIIVATTHQLLTFYKAFDLLIIDEVDAFPFVTNVQLNHAANQASKTDAARILLTATST
TTLEKQVKRGEVEKLTLARRFHNHPLVIPQFIRSFAILNNIHCHKIPEIVIKYLREQRQTGYPLLIFLPVITTAEIVTNL
LKKAFPKEKIACVSSQAEEREKDITAFRQGEKTILVTTTILERGVTFPGVDVFVLAAHHRVFTPQSLVQISGRVGRSLER
PTGNLYFFHDGISRAMLKARKEIKEMNQKGYPDDMPTVPTT

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=404866 GE024_RS08125 WP_093999505.1 1623549..1624874(-) (comFA/cflA) [Streptococcus canis strain HL_100]
ATGGAAGGTATTGAAAATTATTACGGTCGTTTATTTTTAGAAAATCAATTGCCAGAAGAAGGAAAGCATCTTGCAAAACC
TTTAGAAAGTATAGTAATTACAAAAGGTAAGGTTACTTGCCAACGATGCCGTTATCACATTAGTGAGGAAGAAAGATTGC
CTAGTGGCGCTTATTATTGCCGCTTCTGTCTTGTCTTTGGCCGCAATCAATCTGATAAATCGCTTTACTATATGTCCCCT
AAGCCATTCCCTCAAGGAAGCTGTCTTAAATGGAAAGGACAATTAACACCTCATCAAAAAAAGATTTCCCAACAGTTGGT
AAGAAATGTACAGGCCAAAAAGCCTACCTTAGTTCATGCTGTAACAGGAGCTGGTAAAACAGAAATGATTTATGCAGCTA
TTGCAAGAGTTATTGAAGCAGGTGGCTGGGTATGTTTAGCTAGCCCGCGAGTGGATGTTTGTATCGAAGTGGCCAAACGG
TTATCTCAAGCCTTTTCGTGTCAAGTCTGTTTAATGCATGCTGGATCCTCTCCTTACCAGAGAAGTCCTATTATTGTAGC
AACAACACATCAGTTACTCACTTTTTACAAGGCTTTTGACCTTTTGATTATTGATGAAGTTGATGCTTTTCCATTTGTGA
CAAACGTTCAACTGAATCACGCTGCAAATCAAGCCTCTAAAACAGATGCTGCTAGGATTTTATTAACAGCAACATCAACG
ACAACCTTAGAAAAACAAGTCAAAAGAGGAGAAGTAGAAAAGCTAACATTAGCTAGGCGCTTTCATAATCATCCCTTAGT
TATTCCACAGTTTATCAGAAGTTTTGCTATCTTGAACAATATACACTGCCATAAGATTCCTGAAATAGTCATCAAATACC
TTCGAGAGCAGCGGCAGACAGGTTATCCCCTGTTAATATTTCTTCCTGTTATTACAACAGCAGAAATAGTCACCAATTTG
TTAAAAAAAGCTTTTCCAAAAGAAAAGATAGCTTGTGTTTCAAGTCAAGCAGAAGAGAGGGAGAAAGATATTACTGCCTT
TCGCCAGGGTGAAAAAACTATTTTAGTCACGACAACAATCTTGGAAAGGGGGGTTACCTTTCCTGGTGTTGATGTCTTTG
TACTAGCAGCCCACCATCGAGTTTTCACCCCACAAAGTCTTGTTCAAATCTCGGGACGCGTGGGAAGATCTCTGGAGAGA
CCGACAGGGAACTTATACTTTTTTCATGATGGCATCAGTAGAGCAATGTTGAAGGCTCGAAAAGAAATCAAAGAAATGAA
TCAGAAAGGTTACCCAGATGACATGCCTACTGTGCCAACAACCTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(287-396)

DEAD/DEAH box helicase (DEAD)

(98-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3P5Y1B1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

55.294

96.372

0.533
  comFA/cflA Streptococcus pneumoniae D39

55.294

96.372

0.533
  comFA/cflA Streptococcus pneumoniae R6

55.294

96.372

0.533
  comFA/cflA Streptococcus pneumoniae TIGR4

55.294

96.372

0.533
  comFA/cflA Streptococcus mitis NCTC 12261

54.118

96.372

0.522
  comFA/cflA Streptococcus mitis SK321

53.63

96.825

0.519
  comFA Lactococcus lactis subsp. cremoris KW2

45.476

97.732

0.444
  comFA Latilactobacillus sakei subsp. sakei 23K

39.558

92.29

0.365

Multiple sequence alignment